Abstract: In vitro biomimetic models of the neonatal immune system are provided along with methods of using the models in pre-clinical assessment of infant immune cell-mediated and humoral responses to immunogenic stimulation, such as vaccination. The models include one comprising cord blood-derived T follicular helper cells and B cells, and one comprising cord blood-derived dendritic cells and CD4+ T cells. The models can be used, for example, to assess candidate vaccines via analysis of cellular responses to antigen and vaccine exposure.
Type:
Application
Filed:
September 7, 2017
Publication date:
June 20, 2019
Applicant:
Sanofi Pasteur VaxDesign Corporation
Inventors:
Evan GOMES, Tirumalai KAMALA, Luis MOSQUERA, Vaughan WITTMAN, William WARREN, Janice MOSER, Donald DRAKE
Abstract: A system and method provide for high through put determination of agglutination states. The system includes a rotating table and multiple plate tilting stations. The system also includes one or more optical paths positioned to image entire plate arrays in tilted and/or untilted configurations. The system preferably includes image analysis software to analyze an image of an array of test wells and determine an agglutination state of each well based on the image analysis.
Abstract: The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
Type:
Grant
Filed:
September 10, 2012
Date of Patent:
May 13, 2014
Assignees:
Sanofi Pasteur Vaxdesign Corporation, Mount Sinai School of Medicine, The Whitehead Institute, Massachusetts Institute of Technology, The Scripps Research Institute
Inventors:
William L Warren, Heather Fahlenkamp, Russell Higbee, Anatoly Kachurin, Conan Li, Mike Nguyen, Robert Parkhill, Guzman Sanchez-Schmitz, Darrell J. Irvine, Gwendalyn J. Randolph, Nir Harcohen, Bruce Torbett
Abstract: The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the tissues of the immune system in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates and other materials in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system, coupled with disease models to provide a more complete representation of an immune response.
Type:
Grant
Filed:
September 20, 2011
Date of Patent:
October 30, 2012
Assignee:
Sanofi Pasteur Vaxdesign Corporation
Inventors:
William L. Warren, Heather Fahlenkamp, Russell G. Higbee, Eric M. Mishkin, Guzman Sanchez-Schmitz, Michael D. Rivard, Santosh Pawar
Abstract: The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.
Type:
Grant
Filed:
March 24, 2010
Date of Patent:
October 30, 2012
Assignee:
Sanofi Pasteur Vaxdesign Corporation
Inventors:
William L. Warren, Robert Parkhill, Michael N. Nguyen, Guzman Sanchez-Schmitz, Heather Fahlenkamp, Russell Higbee, Donald Drake, III, Anatoly Kachurin, David Moe