Abstract: The invention provides a gas detection system and a method with eliminating influence of ambient temperature and humidity change, the gas detection system comprises a bare sensor, a reference sensor and a calculation module; the bare sensor is used to detect a target gas in an ambient gas to obtain a first feedback signal; the reference sensor is used to selectively isolate the target gas in the ambient gas to produce a zero gas and to detect the zero gas to obtain a second feedback signal, the calculation module is used to calculate a difference between the first feedback signal and the second feedback signal to obtain a third feedback signal, and to obtain a target gas concentration by calculating the third feedback signal according to a calibration formula. The invention improves the measurement accuracy to the target gas concentration, which are efficient and reliable and have good technical effects.