Patents Assigned to Sapporo Medical University
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Publication number: 20140234335Abstract: Disclosed is a method of diagnosing graft-versus-host disease, comprising measuring the level of CCL8 protein in a sample obtained from a subject as an indicator for the diagnosis or course of graft-versus-host disease. Also a diagnostic reagent for graft-versus-host disease comprising an anti-CCL8 antibody is disclosed. The method of the present invention enables diagnosis of the onset of graft-versus-host disease and monitoring of the progress, in particular, differential diagnosis between graft-versus-host disease and an infectious disease. The present invention also provides a method for treating graft-versus-host disease utilizing the anti-CCL8 antibody.Type: ApplicationFiled: April 3, 2014Publication date: August 21, 2014Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Tsukasa HORI, Yasuo KOKAI, Yasuyoshi NAISHIRO, Hiroyuki TSUTSUMI, Kohzoh IMAI
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Publication number: 20140057845Abstract: In order to provide a composition for treating, improving, or preventing pain, it has been found out that a metallothionein protein is expressed in a normal peripheral nerve, and that the expression is not observed in a peripheral nerve producing pain. Moreover, it has been found out that administering metallothionein to rats having neuropathic pain suppresses the pain in the rats.Type: ApplicationFiled: February 23, 2012Publication date: February 27, 2014Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Yasuo Kokai, Gousuke Ooki, Kayo Matsumoto, Shinichi Imai, Takuro Wada
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Publication number: 20140044686Abstract: A molecular marker for detecting a cancer stem cell in a cell mass which is a subject of interest, wherein the molecular marker can be detected in a cancer stem cell contained in the subject of interest but cannot be detected in a normal cell and a cancer cell that is different from a cancer stem cell; a method for determining the presence or absence of a cancer stem cell in a subject of interest by using the molecular marker as an measure; a kit for determining the presence or absence of a cancer stem cell, which comprises at least a reagent for detecting the molecular marker; a polypeptide encoded by the molecular marker; an antibody capable of recognizing an epitope of an expression product of a gene derived from the molecular marker; a nucleic acid capable of inhibiting the expression of the molecular marker; and a nucleic acid vaccine comprising a gene derived from the molecular marker.Type: ApplicationFiled: August 15, 2013Publication date: February 13, 2014Applicants: Dainippon Sumitomo Pharma Co., Ltd., Sapporo Medical UniversityInventors: Toshihiko Torigoe, Yoshihiko Hirohashi, Noriyuki Satoh, Kenjiro Kamiguchi, Rena Morita, Satoshi Nishizawa, Akari Takahashi
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Publication number: 20130323763Abstract: The present invention provides a useful examination method and diagnostic reagent, which are capable of diagnosing acute lung injury in clear distinction from chronic progressive interstitial pneumonia. Specifically, the present invention provides a method of determining whether or not a test subject is affected with acute lung injury, which comprises quantifying heat shock protein 47 in a biological sample derived from the test subject.Type: ApplicationFiled: February 10, 2012Publication date: December 5, 2013Applicants: SAPPORO MEDICAL UNIVERSITY, NAGASAKI UNIVERSITYInventors: Tomoyuki Kakugawa, Shin-ichi Yokota
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Publication number: 20130302838Abstract: A novel biomarker for diagnosing amyloid ?-related neuropathy such as Alzheimer's disease is provided. Disclosed is a method for diagnosing Alzheimer's disease comprising measuring the level of a MFG-E8 protein in a blood, serum or plasma sample obtained from a human or animal subject, wherein the measured value is used as a marker for diagnosis of Alzheimer's disease. The MFG-E8 protein is found to bind to phosphatidylserine in a Ca2+ dependent manner in a culture supernatant of mouse brain nerve cells, which are a dementia model stimulated with A?42. It is also found that the level of the MFG-E8 protein is increased in patients with Alzheimer's disease.Type: ApplicationFiled: December 5, 2011Publication date: November 14, 2013Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Yasuo Kokai, Hitoshi Sohma, Shinichi Imai, Kayo Matsumoto, Michitoshi Kimura
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Publication number: 20130267430Abstract: Disclosed is a method of diagnosing graft-versus-host disease, comprising measuring the level of CCL8 protein in a sample obtained from a subject as an indicator for the diagnosis or course of graft-versus-host disease. Also a diagnostic reagent for graft-versus-host disease comprising an anti-CCL8 antibody is disclosed. The method of the present invention enables diagnosis of the onset of graft-versus-host disease and monitoring of the progress, in particular, differential diagnosis between graft-versus-host disease and an infectious disease. The present invention also provides a method for treating graft-versus-host disease utilizing the anti-CCL8 antibody.Type: ApplicationFiled: June 14, 2012Publication date: October 10, 2013Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Tsukasa HORI, Yasuo KOKAI, Yasuyoshi NAISHIRO, Hiroyuki TSUTSUMI, Kohzoh IMAI
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Publication number: 20130267965Abstract: A ligator for ligating body duct(s), said ligator enabling quick, easy and damage-free repetition of ligation and release of one or more body ducts in a body cavity through an operation outside the body cavity, and having an excellent performance of blocking the flow of a body fluid such as blood or lymph in a body duct. In an abdominal surgery, the ligator can be provided at such a position as not disturbing the surgery per se or hindering the vision during the surgery, while leaving the back-end thereof outside the body cavity, and can be operated outside the body cavity. In an endoscopic surgery, the ligator can be inserted from an opening that is a surgical wound while leaving the back-end thereof outside the body cavity, and can be operated outside the body cavity.Type: ApplicationFiled: October 5, 2011Publication date: October 10, 2013Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Toru Mizuguchi, Koichi Hirata, Masaki Kawamoto, Makoto Meguro
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Patent number: 8541544Abstract: A molecular marker for detecting a cancer stem cell in a cell mass which is a subject of interest, wherein the molecular marker can be detected in a cancer stem cell contained in the subject of interest but cannot be detected in a normal cell and a cancer cell that is different from a cancer stem cell; a method for determining the presence or absence of a cancer stem cell in a subject of interest by using the molecular marker as an measure; a kit for determining the presence or absence of a cancer stem cell, which comprises at least a reagent for detecting the molecular marker; a polypeptide encoded by the molecular marker; an antibody capable of recognizing an epitope of an expression product of a gene derived from the molecular marker; a nucleic acid capable of inhibiting the expression of the molecular marker; and a nucleic acid vaccine comprising a gene derived from the molecular marker.Type: GrantFiled: October 27, 2009Date of Patent: September 24, 2013Assignees: Dainippon Sumitomo Pharma Co., Ltd., Sapporo Medical UniversityInventors: Toshihiko Torigoe, Yoshihiko Hirohashi, Noriyuki Satoh, Kenjiro Kamiguchi, Rena Morita, Satoshi Nishizawa, Akari Takahashi
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Publication number: 20130243736Abstract: In vitro 2-dimensional co-culture systems of LT-HSCs and osteoblastic niche cells and methods of establishing them are provided. For example, in certain aspects methods of screening people suffering from a disorder caused by dysfunction of osteoblastic niche cells, using said co-culture systems are described. In further aspects, methods for screening a candidate substance for treatment of a disorder caused by dysfunction of osteoblastic niche cells are provided. The present invention also concerns methods and therapeutic compositions of treating a patient suffering from a disorder caused by dysfunction of osteoblastic niche cells.Type: ApplicationFiled: January 28, 2013Publication date: September 19, 2013Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Mineko Fujimiya, Koji Ataka, Hironori Chiba
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Patent number: 8481497Abstract: [PROBLEMS] To provide a technique which enables an effective antibody therapy for cancer which targets for FGFR1 without the need of using any effective antibody having high specificity and a potent cell-killing activity. [MEANS FOR SOLVING PROBLEMS] Disclosed are: a therapeutic agent for cancer, which comprises an enhancer of the expression of a fibroblast growth factor receptor-1 and an anti-fibroblast growth factor receptor-1 antibody; and a method for the treatment of cancer using the therapeutic agent.Type: GrantFiled: May 29, 2008Date of Patent: July 9, 2013Assignees: Sapporo Medical University, The University of TokyoInventors: Kohzoh Imai, Shigeru Sasaki, Tsutomu Seito
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Publication number: 20130130382Abstract: To provide an autoserum-containing bone marrow cell culture system, whereby bone marrow cells, which are collected from a subject without using an anticoagulant, are subjected to an anticoagulation treatment using a medium in a liquid-tight state, cultured and then further cultured using the serum of said subject which is prepared in a liquid-tight state; an autoserum-containing bone marrow cell culture method; and a method for producing a medicinal composition which comprises, as the active ingredient, autoserum-containing cultured bone marrow cells. [Solution] An autoserum-containing bone marrow cell culture system for culturing bone marrow cells, which are collected from a subject without using an anticoagulant, using the serum of said subject, said system comprising a bone marrow cell suspension-storing device, a collected blood-storing device, an autoserum-acquiring device, and a bone marrow cell-culturing device.Type: ApplicationFiled: August 3, 2011Publication date: May 23, 2013Applicant: SAPPORO MEDICAL UNIVERSITYInventors: Osamu Honmou, Yoshihiro Yoshikawa, Naomi Morikawa
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Patent number: 8406514Abstract: An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.Type: GrantFiled: December 19, 2008Date of Patent: March 26, 2013Assignees: Nikon Corporation, Sapporo Medical UniversityInventors: Tomotaka Shinoda, Toshihiro Mitaka, Satoshi Mori, Naoki Watanabe, Hideki Ito
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Publication number: 20120264120Abstract: An object of the present invention is to provide a method for non-invasively diagnosing invasiveness or degree of invasion of colorectal tumors. The present invention is characterized in that it enables to obtain a specimen that can be used to detect invasive colorectal tumors by spraying a washing fluid onto the colonic mucous layer of a subject to detach the mucus from the mucous layer, and collecting the detached mucus together with the washing fluid.Type: ApplicationFiled: February 28, 2012Publication date: October 18, 2012Applicant: SAPPORO MEDICAL UNIVERSITYInventors: MINORU TOYOTA, Mutsumi Toyota, EIICHIRO YAMAMOTO, SEIKO KAMIMAE, SUZUKI HIROMU, HIROO YAMANO
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Publication number: 20120255853Abstract: A method for easily and stably producing a superoxide by selectively generating the superoxide or a radical containing the superoxide at a high purity; a method for easily evaluating the superoxide scavenging ability of a subject sample; a device for easily and stably producing a superoxide by selectively generating the superoxide or a radical containing the superoxide at a high purity; and a device for easily evaluating the superoxide scavenging ability of a subject sample are provided. The method for producing a superoxide includes: a step (a) for preparing a solution for determination; a step (b) for forming a spin adduct/radical for determination; a step (c) for acquiring a spectrum for determination; a step (d) for determining similarity; a step (e) for acquiring flavin concentration; a step (f) for preparing a starting material solution; and a step (g) for generation.Type: ApplicationFiled: December 8, 2010Publication date: October 11, 2012Applicant: Sapporo Medical UniversityInventors: Fujii Hirotada, Shunji Kohri
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Publication number: 20120237518Abstract: The present invention relates to a monoclonal antibody or an antibody fragment thereof, which binds to the extracellular region of human Trop-2 with high affinity and exhibits high ADCC activity and high antitumor activity; a hybridoma which produces the antibody; a DNA which encodes the antibody; a vector which comprises the DNA; a transformant obtainable by introducing the vector; a process for producing an antibody or an antibody fragment thereof using the hybridoma or the transformant; and a therapeutic agent or a diagnostic agent using the antibody fragment thereof.Type: ApplicationFiled: June 10, 2011Publication date: September 20, 2012Applicants: KYOWA HAKKO KIRIN CO., LTD, SAPPORO MEDICAL UNIVERSITYInventors: Miki YAMAGUCHI, Kazunori Kato, Hirofumi HAMADA, Kazuyasu NAKAMURA, Yoshiyuki SUGIMOTO, Tsuguo KUBOTA, Masahiro IKEDA
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Patent number: 8232069Abstract: The present invention aims at developing a gene delivery system which has a high selectivity to a target cell and can introduce and express a gene with high efficiency, particularly developing such a system for use in a gene delivery therapy using a viral vector. The present invention provides a method for targeting a drug, which comprises the step of delivering a drug containing a therapeutic gene to a target site using an anti-PAP2a antibody.Type: GrantFiled: May 17, 2006Date of Patent: July 31, 2012Assignee: Sapporo Medical UniversityInventors: Hirofumi Hamada, Kiminori Nakamura, Kazunori Kato
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Patent number: 8216152Abstract: PROBLEMS TO BE SOLVED: A finger arterial elasticity measuring program, a finger arterial elasticity measuring device and a finger arterial elasticity measuring method are provided for making it possible to measure an elasticity index of a finger artery in accordance with a pulse wave of a finger artery without measuring a blood pressure in evaluating the elasticity of the finger artery related to the degree of arterial sclerosis in an easy and least expensive manner.Type: GrantFiled: February 12, 2008Date of Patent: July 10, 2012Assignee: Sapporo Medical UniversityInventors: Gohichi Tanaka, Yukihiro Sawada
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Publication number: 20120021063Abstract: To provide a collagen/elastin crosslinked material capable of satisfactorily corresponding to various uses such as medical materials including an artificial dermis and scaffolding materials for cell culture. The collagen/elastin crosslinked material is obtained by crosslinking a fish-derived collagen and a fish-derived elastin, and is fully capable of corresponding to various uses such as medical materials including an artificial dermis and scaffold material for cell culture.Type: ApplicationFiled: March 10, 2010Publication date: January 26, 2012Applicants: MARUHA NICHIRO FOODS, INC., HIROSAKI UNIVERSITY, SAPPORO MEDICAL UNIVERSITYInventors: Yoshitaka Matsumoto, Takatoshi Yotsuyanagi, Shinichi Suto, Yoshifumi Yamaya, Yosuke Hoshino, Kazunari Nishimura, Tomoko Koga, Hiroyuki Enari
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Publication number: 20110262358Abstract: A molecular marker for detecting a cancer stem cell in a cell mass which is a subject of interest, wherein the molecular marker can be detected in a cancer stem cell contained in the subject of interest but cannot be detected in a normal cell and a cancer cell that is different from a cancer stem cell; a method for determining the presence or absence of a cancer stem cell in a subject of interest by using the molecular marker as an measure; a kit for determining the presence or absence of a cancer stem cell, which comprises at least a reagent for detecting the molecular marker; a polypeptide encoded by the molecular marker; an antibody capable of recognizing an epitope of an expression product of a gene derived from the molecular marker; a nucleic acid capable of inhibiting the expression of the molecular marker; and a nucleic acid vaccine comprising a gene derived from the molecular marker.Type: ApplicationFiled: October 27, 2009Publication date: October 27, 2011Applicants: Sapporo Medical University, Dainippon Sumitomo Pharma Co., Ltd.Inventors: Toshihiko Torigoe, Yoshihiko Hirohashi, Noriyuki Satoh, Kenjiro Kamiguchi, Rena Morita, Satoshi Nishizawa, Akari Takahashi
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Publication number: 20110158908Abstract: Disclosed are: a composition and a method for the diagnosis and/or the treatment of a CSABP-related disease, the regulation of the proliferation of an RNA virus and the regulation of an RNA-metabolizing system, which targets a cyclosporin A-binding protein (CSABP); a method for the screening of a component that targets a CSABP; a composition and a method for the detection/inhibition of CsA, NS5B or cyclosphilin B by utilizing a CSABP; a promoter for a CSABP; a method for the screening of a substance capable of regulating the expression of a CSABP by utilizing the promoter; and a method for the determination of the occurrence or clinical stage of a CSABP-related disease.Type: ApplicationFiled: August 22, 2008Publication date: June 30, 2011Applicant: Sapporo Medical UniversityInventors: Hiroeki Sahara, Yoko Mori, Nobuaki Takahashi, Noriyuki Sato, Fumio Sugawara, Kengo Sakaguchi, Kengo Morohashi, Kazuki Iwabata, Koichi Watashi, Kunitada Shimotohno, Kokichi Kikuchi, Wataru Tsutae, Hiroki Miyashita