Abstract: The invention discloses a method for producing a single cell oil containing gamma-linolenic acid characterized in that at least one microorganism of the order Mucorales preferably of the genus Mortierella, Actinomucor, Mucor, Rhizomucor or Rhizopus is cultured in a growth medium which is substantially starch and sugar free and which contains, as a carbon source material, at least one monocarboxylic acid of 2 to 5 carbon atoms, preferably acetic acid and recovering the oil from the resultant cultured microorganism biomass. The invention also relates to a method of treating the organic acid stream of the Fischer-Tropsch synthesis process to remove organic material therefrom.