Abstract: The invention relates to at least one nucleotide sequence, derived from a nucleotide sequence encoding an acyltransferase polypeptide comprising at least one membrane-spanning region, encoding an improved active membrane independent acyltransferase polypeptide in which at least one amino acid residue of the membrane-spanning region has been deleted and/or substituted as compared to the original acyltransferase polypeptide, wherein the encoded active membrane independent acyltransferase polypeptide can produce fatty acid esters and/or fatty acid thioesters such as triacylglycerols, diacylglycerols, monoacylglycerols, phospholipids, glycolipids, waxesters, acylated carbohydrates, acylated amino acids, and lysolipids, e.g. lysophosphospholipid, lysolecithin. Thereby one single acyltransferase can be used for the production of a huge number of products.
Type:
Application
Filed:
May 28, 2003
Publication date:
June 29, 2006
Applicant:
SCANDINAVIAN BIOTECHNOLOGY RESEARCH (ScanBi) AB
Inventors:
Ylva Lindqvist, Antoni Banas, Anders Dahlqvist, Alokesh Ghosal
Abstract: The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-CoA: diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil (i.e. triacylglycerols) that is produced.
Type:
Application
Filed:
May 26, 2004
Publication date:
January 6, 2005
Applicant:
SCANDINAVIAN BIOTECHNOLOGY RESEARCH (SCANBI) AB
Inventors:
Antoni Banas, Line Sandager, Ulf Stahl, Anders Dahlqvist, Marit Lenman, Hans Ronne, Sten Stymne
Abstract: The present invention relates to the use of a novel enzyme and its encoding gene for transformation. More specifically, the invention relates to the use of a gene encoding an enzyme with acyl-CoA:diacylglycerol acyltransferase activity. This gene expressed alone in transgenic organisms will increase the total amount of oil (i.e. triacylglycerols) that is produced.
Type:
Grant
Filed:
November 13, 2000
Date of Patent:
September 14, 2004
Assignee:
Scandinavian Biotechnology Research (ScanBi) AB
Inventors:
Antoni Banas, Line Sandager, Ulf Ståhl, Anders Dahlqvist, Marit Lenman, Hans Ronne, Sten Stymne