Abstract: Access to microscope images while a sample is scanned or images are generated or uploaded can decrease an amount of time that a user waits to view a region of interest of an image. A processor can be configured to allow a remote user to access a portion of an image at full or partial resolution while other portions of the sample are being scanned, or while one or more images are generated or uploaded at full or partial resolution. A processor stored locally with the microscope can be configured to allow a remote user to access a completed scan over the internet prior to the scan being fully uploaded over a network such as the internet to a remote server. In some embodiments, a processor may be coupled to a microscope, a user device, or a remote server.

Abstract: Disclosed herein are systems and methods for constructing an image of a sample using a plurality of images acquired under multiple illumination conditions. In some cases, a microscope may include an image capture device, an illumination assembly, and a processor configured to acquire a plurality of images of a sample and a fiducial marker under a plurality of different illumination conditions and to reconstruct a high resolution image in response to the plurality of images. The disclosure also provides a method for generating a high resolution image of a sample comprising acquiring a plurality of images of a sample and a fiducial marker under a plurality of different illumination conditions and reconstructing the high resolution image in response to the plurality of images.