Abstract: The methods have application to the tissue culture of plant cells, ovules and microspores. Automated imaging, automated cell tracking and predictive modeling are used to develop methods that predict the likelihood that a plant cell will develop into a desired phenotype and/or which cellular reprogramming factors will assist in this development. The methods taught herein can also be used to evaluate the toxicity effects of compounds on plant cells, predict genotypic responses to tissue culture and cellular reprogramming factors, determine cell ploidy status, and predict other types of cell phenotype development.

Abstract: The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.

Abstract: The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.

Abstract: The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells.