Abstract: The present disclosure relates to a complementary double-stranded oligo, in which, for the amplification of a particular gene sequence, an inosine linker is linked to the 5?-terminus of a primer for the corresponding sequence, a sequence complementary to the primer is linked to the inosine linker, and at least one mis-matched nucleotide is included in the complementary sequence to form a bubble structure; in which, depending on the treatment temperature, at a predetermined temperature or lower, a single stranded oligo is turned into a double-stranded form to exist in an inactivation form, and at a predetermined temperature or higher, the oligo is activated into a single-stranded oligo; and in which, a fluorescent substance (reporter dye) and a quenching material (quencher molecule) are attached to the oligo, so that the oligo can be applied as a primer or a probe, and thus only two oligos can realize the gene amplification and fluorescent signal real-time measurement with high specificity, and to a measuring

Abstract: Provided is a genome extraction device including a flow cover, more particularly a genome extraction device which includes a base plate, thereby forming a closed flow path inside and preventing a problem of narrowing of the flow path according to close contact coupling between components.

Abstract: The present invention relates to a glucose diffusion control layer solution for a continuous blood glucose-measuring biosensor, a glucose diffusion control layer for a continuous blood glucose-measuring biosensor, comprising the same, and a continuous blood glucose-measuring biosensor comprising the same. More specifically, the present invention relates to: a glucose diffusion control layer solution for a continuous blood glucose-measuring biosensor, in which, by using only a substance of which biocompatibility has been verified, stability can be ensured even when injected into the human body, and glucose permeability and water absorbance can be controlled; a glucose diffusion control layer for a continuous blood glucose-measuring biosensor, comprising the same; and a continuous blood glucose-measuring biosensor comprising the same.

Abstract: Provided is a genome extraction device to which a dual chamber structure of an outer chamber and an inner chamber is applied, more particularly a genome extraction device to which the above-described dual chamber structure is applied so that the genome extraction device can be stored stably for a long time without the risk of reagent leakage.

Abstract: Provided is an amplification module with a gas moving passage and an extract moving passage, more particularly an amplification module in which, when an extract is input from a genome extraction device in which the amplification module is installed, a quantitative amount of the extract is input to each accommodating portion so that the accuracy of detection can be increased.

Abstract: Provided is a genome extraction device to which a dual chamber structure of an outer chamber and a bead chamber is applied, more particularly a genome extraction device to which the above-described dual chamber structure is applied so that the performance of dry beads vulnerable to moisture can be maintained for a long time.

Abstract: Provided is a diagnostic kit, in which a detection device and a specimen collection tool are integrated. The diagnostic kit includes a specimen collector capable of collecting a specimen, a device including a housing and a detection strip, and a connector connecting the specimen collector with the device and moving a solution, the connector having a bar shape.

Abstract: The invention relates to an isothermal-based dual functional oligonucleotide containing quencher and reporter dye for an isothermal nucleic acid amplification and a method for nucleic acid amplification and measurement using the same. The present invention is directed to a method capable of obviating the need for an additional oligonucleotide, in addition to four to six types of oligonucleotides for a nucleic acid amplification reaction of LAMP, detecting the amount of fluorescence according to the amplification of the nucleic acid of target gene—specific sequence for DNA and RNA, enabling the detection also after the completion of the reaction, and detecting the amount of fluorescence in real-time. Therefore, the present invention allows simultaneous multiple tests by measuring the amount of fluorescence in one tube after the completion of the reaction or in real time while varying the reporter dye according to the target gene.

Abstract: There is provided a nucleic acid extraction method using a cartridge comprising: (a) a driving part of a nucleic acid extraction device is connected to a control rod module disposed in an inner space of the upper body of a piston and a rotation control module coupled to the lower body of the piston; (b) driving the rotation control module and the control rod module, sequentially sucking sample and reagents from the plurality of chambers separated from each other into an interior space, and discharging the mixture of the interior space into the chamber of the cartridge; and (c) driving the rotation control module and the control rod module to suck the reagent inside the master mix bead chamber of the cartridge into the interior space of the piston upper body and then discharge the mixed reagent to a nucleic acid amplification module.

Abstract: There is provided a cartridge for nucleic acid extraction comprising: a first body having a plurality of chambers in which ports are formed at the bottom; a second body coupled to a lower region of the first body; and a piston disposed rotatably in the centers of the first body and the second body and having a port formed at the bottom thereof; and characterized in that the cartridge comprises a plurality of flow paths formed on the upper region of the second body, one end overlapping the port of the piston and the other end overlapping the port of the first body.

Abstract: The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5?-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5?-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.