Abstract: A method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner is disclosed. A method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein is also disclosed. It has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Abstract: An objective of the present invention is to provide a culturing method, by which Bacillus bacterial spores can be efficiently produced. The present invention provides a method for producing Bacillus bacterial spores, comprising culturing Bacillus bacteria in a liquid medium containing a sugar or a sugar-source raw material at a concentration between 50.1 g/L and 100 g/L at the start of culture, and then in the course of the culture, feeding a fed-batch medium containing a sugar or a sugar-source raw material and a nitrogen-containing compound, and having the weight ratio (C/N ratio) of carbon atoms to nitrogen atoms ranging from 5.5 to 13.5 to the liquid medium.

Abstract: In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Abstract: The present invention provides a process for producing a compound represented by formula (I), comprising the steps of (a) reacting a compound represented by formula (II) with dimethyl sulfate in the presence of an alkali carbonate in a aqueous ketone solvent to obtain the compound represented by formula (I) as a crystalline material, and (b) washing the crystalline material with heated water at 30 to 100° C. and then further washing with an organic solvent at 30 to 80° C.

Abstract: An object of the present invention is to provide a novel culturing method by which spores can be efficiently produced. The present invention further provides a method for culturing sporulating bacteria, comprising adding a sporulation-inhibiting substance into a medium for culturing sporulating bacteria, wherein the carbon content in the medium is 9.1 g/L or more, and preferably further comprising a step of adding a sporulation-accelerating substance to the medium.

Abstract: The present invention aims to provide a plant growth regulating agent for improving environmental stress resistance of a plant, for growth promotion of a plant, or for antimicrobial purpose in a plant, and provides a plant growth regulating agent for improving environmental stress resistance of a plant, for growth promotion of a plant, or for antimicrobial purpose in a plant, the plant growth regulating agent including hesperidin or a hesperidin derivative, or a flavonoid, as an effective component.

Abstract: The present invention relates to a plant disease control agent containing a compound represented by the following formula (1) or salt thereof as an active ingredient (in the formula, R1 and R2 represents a hydrogen atom or methyl group but excepting cases where R1 and R2 are the same), a plant disease controlling method applying the plant disease control agent on plants suffering from diseases, and a novel compound represented by formula (1) or salt thereof.

Abstract: In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Abstract: By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.

Abstract: A microorganism selected from the group consisting of a Bacillus sp. ITB090 strain (NITE BP-01725), a Bacillus sp. ITB100 strain (NITE BP-01726), and a Bacillus sp. ITB105 strain (NITE BP-01727), or a variant strain derived therefrom is used to control plant diseases, control nematodes, promote plant growth, and the like.

Abstract: An agricultural and horticultural fungicide composition including prumycin and a surfactin family member as active components and a plant disease controlling method. As the surfactin, family member, surfactin and [Ile7]surfactin are preferred. The composition preferably further contains calcium chloride comprises for improving storage stability.

Abstract: The present invention provides a novel agricultural and horticultural fungicide composition including prumycin and a surfactin family member as active components and a plant disease controlling method. As the surfactin, surfactin and [Ile7]surfactin are preferred. The present invention provides a practically useful agricultural and horticultural fungicide composition having a high effect and excellent storage stability, which composition further comprises calcium chloride so as to remarkably improve the storage stability thereof.

Abstract: The present invention relates to the Bacillus sp. strains AT-332 (NITE BP-1095) and AT-79 (NITE BP-1094) isolated from nature; and a plant disease control agent, a nematode control agent and a plant growth promoter containing the strains as active bacteria. The Bacillus sp. strains AT-332 and AT-79 strain are effective in controlling both a wide range of various plant diseases and nematode-damage and capable of promoting the growth of useful plants, due to a culture containing a secondary metabolite of the strains or cultivated and isolated live bacteria of the strains being introduced to a plant body or to the culture soil.

Abstract: The present invention relates to a microbial pesticide composition, which is obtained by a method including the steps of: adjusting a pH of a culture solution of the Bacillus sp. bacterium to from 3.0 to 5.0 (pH adjustment step); mixing the culture solution with the calcium chloride and/or the magnesium sulfate (mixing step); and lyophilizing or spray-drying the culture solution (drying step). In the microbial pesticide composition, even if it is stored for a long period of time at room temperature, the active ingredient remains stable and a sufficient preventive effect against plant disease can be maintained.

Abstract: The invention relates to a compound or a salt thereof of formula [1], a fungicidal composition containing the compound or a salt thereof, a method for controlling plant disease occurring from phytopathogenic microorganism including the step of applying the fungicidal composition and a production method of the compound.

Abstract: The invention relates to a compound or a salt thereof of formula [1], a fungicidal composition containing the compound or a salt thereof, a method for controlling plant disease occurring from phytopathogenic microorganism including the step of applying the fungicidal composition and a production method of the compound.

Abstract: The invention relates to a compound or a salt thereof of formula [1], a fungicidal composition containing the compound or a salt thereof, a method for controlling plant disease occurring from phytopathogenic microorganism including the step of applying the fungicidal composition and a production method of the compound.

Abstract: An inhibitor for tobacco axillary bud growth, containing chlorthal-dimethyl and aliphatic alcohol selected from decyl alcohol, 2-ethyl hexanol and geraniol in combination; and a method for inhibiting tobacco axillary bud growth, which includes applying the inhibitor for tobacco axillary bud growth.

Abstract: The present invention relates to the Bacillus sp. strains AT-332 (NITE BP-1095) and AT-79 (NITE BP-1094) isolated from nature; and a plant disease control agent, a nematode control agent and a plant growth promoter containing the strains as active bacteria. The Bacillus sp. strains AT-332 and AT-79 strain are effective in controlling both a wide range of various plant diseases and nematode-damage and capable of promoting the growth of useful plants, due to a culture containing a secondary metabolite of the strains or cultivated and isolated live bacteria of the strains being introduced to a plant body or to the culture soil.

Abstract: The present invention relates to a microbial pesticide composition, which is obtained by a method including the steps of: adjusting a pH of a culture solution of the Bacillus sp. bacterium to from 3.0 to 5.0 (pH adjustment step); mixing the culture solution with the calcium chloride and/or the magnesium sulfate (mixing step); and lyophilizing or spray-drying the culture solution (drying step). In the microbial pesticide composition, even if it is stored for a long period of time at room temperature, the active ingredient remains stable and a sufficient preventive effect against plant disease can be maintained.