Abstract: A method of alleviating central nervous system-mediated pain includes applying salmon thrombin at a neural injury site. Applying salmon thrombin can include applying a gel that includes salmon thrombin. The gel can also include fibrinogen, for example, salmon fibrinogen, human fibrinogen, or bovine fibrinogen. The salmon thrombin can be obtained from salmon plasma, or using recombinant technology, or by fractionation. A pain relief substance includes a gel that includes salmon thrombin.

Abstract: A wound dressing includes a backing material, and thrombin, applied to the backing material. Preferably, the backing material includes biodegradable polyester, such as electrospun polycaprolactone. The thrombin preferably includes salmon thrombin adsorbed onto the backing material. A process of fabricating a wound dressing includes applying thrombin to the backing material. Preferably, the thrombin is prepared from the plasma of a salmonid. The thrombin is applied to the backing material by adsorbing the thrombin onto the backing material, preferably after applying a detergent solution to the backing material. The dressing can be lyophilized and vacuum-sealed within an air-tight wrapping.

Abstract: A method of alleviating pain associated with tissue damage includes applying salmon thrombin at a tissue damage site, as a single substance in liquid form, or as a powder, a foam, and/or a gel that includes salmon thrombin. A pain relief substance includes a salmon thrombin preparation.

Abstract: A method of alleviating pain associated with tissue damage includes applying salmon thrombin at a tissue damage site, as a single substance in liquid form, or as a powder, a foam, and/or a gel that includes salmon thrombin. A pain relief substance includes a salmon thrombin preparation.

Abstract: A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish that is a progeny of domesticated broodstock that are reared under consistent and reproducible conditions, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using the extracted plasma components, and none of any remainder of the plasma, in a nutrient medium. The tissue cultured using the extracted plasma component is other than fish tissue, such as mammalian tissue or insect tissue.

Abstract: A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using the extracted plasma components, and none of any remainder of the plasma, in a nutrient medium. The tissue cultured using the extracted plasma component is other than fish tissue, such as mammalian tissue or insect tissue.

Abstract: A method of using fish ovarian fluid for culture and preservation of mammalian cells includes obtaining ovarian fluid from a fish, and culturing mammalian cells in media including a commercially defined medium and a nutrient medium, wherein the nutrient medium includes the fish ovarian fluid. The cells may initially be cultured in a commercial medium and a conventional nutrient medium prior to culture in the fish ovarian fluid. Living cells may also be preserved by obtaining ovarian fluid from a fish, isolating the living cells, and adding the fish ovarian fluid to the living cells.

Abstract: The present invention is a fibrin sealant and a method for producing a fibrin sealant from fish blood. Whole blood is drawn from the donor fish and centifuged to separate blood cells from plasma. Prothrombin and fibrinogen are extracted from this plasma, and the prothrombin is activated to thrombin. The fibrinogen and thrombin components are then combined as needed to form the fibrin sealant.

Abstract: Method of extracting fish serum and using the fish serum as a blocking reagent for solid phase immunoassays. The donor fish for the serum are taken from domesticated stocks reared under controlled conditions similar to rearing conditions for herds of domestic land animals. Control of genetics, habitat, diet, and reproductive status of the donor fish permits reproducibility of results and also provides batch-to-batch consistency in serum taken from the fish.

Abstract: A method for culturing insect cells using fish serum. The method uses serum extracted from the blood of fish to culture insect cells for various purposes. The technique has the key advantages of consistent quality, low cross reactivity, safety from infectious agents that would endanger researchers or humans and animals receiving cell culture products as therapy and has appropriate nutrients to maintain the growth of insect cells. Fish serum is used together with designated defined medium to allow insect cells to grow and populations to be maintained. The method may also be used with lumpfish to prevent the attachment of insect cells to cultureware. Serum is derived from the blood of captive stocks of fishes raised under control conditions.

Abstract: A method for culturing mammalian cells using fish serum. The method uses serum extracted from the blood of fishes to culture mammalian cells for various purposes. The technique has the key advantages of 1) freedom from mammalian infectious agents that could contaminate cell lines or endanger researchers or recipients of therapeutants derived from mammalian cell culture; 2) consistent and reproducible serum content and quality; 3) low cross-reactivity; and 4) provision of the appropriate serum nutrients to maintain the growth of mammalian cells. Fish serum is used together with designated defined media to allow mammalian cells to grow and populations of these cells to be maintained. Blood serum is derived from captive stocks of fish raised under controlled conditions such that the diet, habitat, genetics, life history, and reproductive status of the fish remains substantially consistent and reproducible.

Abstract: A method for culturing mammalian cells using fish serum. The method uses serum extracted from the blood of fishes to culture mammalian cells for various purposes. The technique has the key advantages of 1) freedom from mammalian infectious agents that could contaminate cell lines or endanger researchers or recipients of therapeutants derived from mammalian cell culture; 2) consistent and reproducible serum content and quality; 3) low cross-reactivity; and 4) the appropriate serum nutrients to maintain the growth of mammalian cells. Fish serum is used together with designated defined media to allow mammalian cells to grow and populations of these cells to be maintained. Blood serum is derived from captive stocks of fish raised under controlled conditions such that the diet, habitat, genetics, life history, and reproductive status of the fish remains substantially consistent and reproducible.

Abstract: A method for culturing insect cells using fish serum. The method uses serum extracted from the blood of fish to culture insect cells for various purposes. The technique has the key advantages of consistent quality, low cross reactivity, safety from infectious agents that would endanger researchers or humans and animals receiving cell culture products as therapy and has appropriate nutrients to maintain the growth of insect cells. Fish serum is used together with designated defined medium to allow insect cells to grow and populations to be maintained. The method may also be used to prevent the attachment of insect cells to cultureware. Serum is derived from the blood of captive stocks of fishes raised under control conditions.