Abstract: The invention provides vaccination protocols for administering immunogens to a primate host in order to promote the formation of neutralizing antibodies (NAbs) against primate immunodeficiency viruses. In some embodiments, the vaccination protocols comprise the step of administering to a primate host a first immunogen comprising at least one primate immunodeficiency virus Envelope (env) sequence having a first set of consensus glycosylation sequences, followed by a second immunogen comprising at least one primate immunodeficiency virus env sequence having a second set of consensus glycosylation sequences, wherein the differences between the first set of consensus glycosylation sequences and the second set of consensus glycosylation sequences comprise differences in consensus glycosylation sequences observed in HIV isolates obtained at different time points of a natural infection.
Type:
Application
Filed:
March 2, 2007
Publication date:
October 30, 2008
Applicants:
Seattle Biomedical Research Institute, University of Washington
Inventors:
Nancy L. Haigwood, Wendy Blay, Leonidas Stamatatos
Abstract: In one aspect, the invention provides an HIV envelope heterotrimer comprising at least two different Env glycoprotein monomers. In some heterotrimers, at least two Env glycoprotein monomers are from different HIV isolates, for example, different HIV-1 isolates. Heterotrimers may contain Env glycoprotein monomers from HIV isolates belonging to the same clade or to different clades or both. At least one of the Env glycoprotein monomers in a heterotrimer of the invention may be modified in a way that alters its amino acid composition. In another aspect, the invention provides methods for inducing an immune response in a vertebrate host against HIV or an HIV-infected cell, comprising administering to a vertebrate host a prophylactically or therapeutically effective amount of a composition comprising an HIV envelope heterotrimer.
Abstract: Method for inoculating a vertebrate host against malaria, by administering to the host a live Plasmodium organism that is genetically engineered to disrupt a liver-stage-specific gene function.
Type:
Application
Filed:
December 20, 2004
Publication date:
February 7, 2008
Applicants:
Seattle Biomedical Research Institute, Ruprecht-Karls-Universitat Heidelberg
Inventors:
Stefan H.I. Kappe, Kai-Uwe C. Matuschewski, Ann-Kristin Mueller
Abstract: Method for inoculating a vertebrate host against malaria, by administering to the host a live Plasmodium organism that is genetically engineered to disrupt a liver-stage-specific gene function.
Type:
Grant
Filed:
August 30, 2006
Date of Patent:
August 28, 2007
Assignees:
Seattle Biomedical Research Institute, Ruprecht-Karls-Universitat Heidelberg
Inventors:
Stefan H. I. Kappe, Kai-Uwe C. Matuschewski, Ann-Kristin Mueller
Abstract: Method for inoculating a vertebrate host against malaria, by administering to the host a live Plasmodium organism that is genetically engineered to disrupt a liver-stage-specific gene function.
Type:
Application
Filed:
August 30, 2006
Publication date:
January 11, 2007
Applicants:
Seattle Biomedical Research Institute, Ruprecht-Karls-Universitat Heidelberg
Inventors:
Stefan Kappe, Kai-Uwe Matuschewski, Ann-Kristin Mueller
Abstract: Method for inoculating a vertebrate host against malaria, by administering to the host a live Plasmodium organism that is genetically engineered to disrupt a gene whose expression is up-regulated in liver stage parasites and whose function is not required for entry into hose hepatocytes.
Type:
Grant
Filed:
March 11, 2005
Date of Patent:
October 17, 2006
Assignees:
Seattle Biomedical Research Institute, Ruprecht-Karls-Universität Heidelberg
Inventors:
Stefan H. I. Kappe, Kai-Uwe C. Matuschewski, Ann-Kristin Mueller
Abstract: Method for inoculating a vertebrate host against malaria, by administering to the host a live Plasmodium organism that is genetically engineered to disrupt a liver-stage-specific gene function.
Type:
Application
Filed:
March 11, 2005
Publication date:
October 13, 2005
Applicants:
Seattle Biomedical Research Institute, Ruprecht-Karls-Universitat Heidelberg
Inventors:
Stefan Kappe, Kai-Uwe Matuschewski, Ann-Kristin Mueller
Abstract: The invention relates to stem cell compositions comprising anti-TGF-&bgr; treated stem cells which are viable for at least 14 days in culture without replication or differentiation and methods for rapid and long term in vitro hematopoiesis and in vivo hematopoietic reconstitution using such anti-TGF-&bgr; treated stem cells.
Type:
Grant
Filed:
January 25, 2000
Date of Patent:
September 30, 2003
Assignee:
Seattle Biomedical Research Institute
Inventors:
Stephen H. Bartelmez, Ewa Sitnicka, Frank Ruscetti