Abstract: The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions.

Abstract: The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions.

Abstract: The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s)with several negative electric charges at an alkaline pH. By way of example this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions.

Abstract: A method for capillary electrophoretic analysis of a biological sample, comprising using negatively surcharged modified antibodies so that they migrate to a zone located outside the migration zone for proteins from the biological sample when they are separated during electrophoresis is disclosed. The antibodies have antigenic specificity for a predetermined target protein.

Abstract: The invention concerns a free solution capillary electrophoresis method at alkaline pH for the analysis of samples comprising protein constituents including a lipoprotein constituent or constituents, characterized in that it comprises at least one step in which the sample is introduced into a capillary tube containing an analysis buffer, said analysis buffer further comprising at least one anionic surfactant type additive that is capable of hydrophobic interaction with the lipoprotein constituent(s) and of modifying the electrophoretic mobility. The invention also concerns a composition for capillary electrophoresis and a kit for analyzing protein constituents.

Abstract: The invention concerns a method for free solution capillary electrophoresis at an alkaline pH to analyze samples comprising haemoglobin, in which the sample is passed through a capillary containing an analysis buffer, comprising at least one step in which the sample is introduced into a capillary tube containing a solution of analysis buffer, characterized in that the buffer is of the zwitterionic type and in that it is associated with at least one flow inhibitor. It also concerns the use of CE flow inhibitors associated with at least one zwitterionic buffer, and a kit for analyzing haemoglobin by capillary electrophoresis.

Abstract: The invention relates to a method for analysis by capillary electrophoresis of glycated haemoglobins comprising at least one globin chain comprising a glucose residue bound to the amino acid in the N-terminal position, contained in a biological sample, said method comprising using a buffer composition comprising at least one compound which is capable of specifically complexing glucose residues of one or several glycated haemoglobin(s) and of providing said glycated haemoglobin(s) with several negative electric charges at an alkaline pH. By way of example, this compound may be 3,4- or 3,5-dicarboxyphenylboronic acid, preferably 3,5-dicarboxyphenylboronic acid. Said method may in particular be used to separate and assay haemoglobin HbA1c present in a biological sample optionally comprising other haemoglobins, in particular other minor fractions.

Abstract: Capillary electrophoresis systems, reagents, and processes for analyzing a sample which includes at least one protein constituent are disclosed. The processes involve introducing the sample into a capillary tube containing a buffer system, wherein said buffer system includes at least one additive having a hydrophobic interaction with said at least one protein constituent and providing said at least one protein constituent with at least one negative charge thereby modifying the electrophoretic mobility.

Abstract: The invention concerns a mask for distributing reagents on an analytical support, such as an electrophoresis gel, the mask having a body with parallel upper and lower surfaces, a plurality of lanes located on the lower surface of the mask have wedge-shaped projecting elements with a sloped surface between first and second ends thereof, an opening extends from the upper surface of the body of the mask to the lowest point of the sloped surface. The mask is positioned over an analytical support and loaded with the desired reagents which are held by capillary action between the mask and the analytical support. The mask can then be moved in a sweeping motion across the analytical support to deposit the reagents thereon. In another embodiment, the projecting elements are rectangular in shape and the mask is positioned at an incline with respect the analytical support.

Abstract: The invention concerns a method for free solution capillary electrophoresis at an alkaline pH to analyze samples comprising haemoglobin, in which the sample is passed through a capillary containing an analysis buffer, comprising at least one step in which the sample is introduced into a capillary tube containing a solution of analysis buffer, characterized in that the buffer is of the zwitterionic type and in that it is associated with at least one flow inhibitor. It also concerns the use of EC flow inhibitors associated with at least one zwitterionic buffer, and a kit for analyzing haemoglobin by capillary electrophoresis.

Abstract: The invention concerns a free solution capillary electrophoresis method at alkaline pH for the analysis of samples comprising protein constituents including a lipoprotein constituent or constituents, characterized in that it comprises at least one step in which the sample is introduced into a capillary tube containing an analysis buffer, said analysis buffer further comprising at least one anionic surfactant type additive that is capable of hydrophobic interaction with the lipoprotein constituent(s) and of modifying the electrophoretic mobility. The method also concerns a composition for capillary electrophoresis and a kit for analyzing protein constituents.

Abstract: The invention concerns a method for capillary electrophoretic analysis of a biological sample, comprising using negatively surcharged modified antibodies so that they migrate to a zone located outside the migration zone for proteins from the biological sample when they are separated during electrophoresis, said antibodies having antigenic specificity for a predetermined target protein.

Abstract: The present invention concerns a presentation an azo dye for staining proteins to allow their analysis, in particular of the amido black type, which is a concentrate in a liquid medium, and to a method for preparing a protein staining solution. It also concerns staining kits comprising said concentrated stain solutions.

Abstract: A device for analyzing samples by electrophoresis comprises a plurality of capillaries, receiver means comprising a multiplicity of independent units each made of a material that is thermally conductive and electrically insulating, and designed to be closely enclosed over the central portion of a capillary, and temperature regulator means comprising at least one Peltier unit for exchanging heat with the units via a solid/solid exchange so as to regulate the temperature of the capillaries via said units.

Abstract: The invention concerns a mask for distributing one or more reagents on an analytical support, in particular an electrophoresis support, for example an electrophoresis gel. The mask (10) (termed a mobile mask) held in a mask holder 12 is designed to be displaced above predetermined zones on an analytical support, and comprises traversing orifices 36 for distributing reagents on the analytical support.

Abstract: The invention concerns a composition for use in a process for separating the constituents of a sample by electrophoresis on an electrophoresis support, comprising one or more ionic compounds which, on applying an electric field to an electrophoresis support having negative surface charges, causes hydration of the zone for loading the sample to be separated when said zone carries a compression mark resulting from loading the sample.

Abstract: The invention concerns a composition for use in a process for separating the constituents of a sample by electrophoresis on an electrophoresis support, comprising one or more ionic compounds which, on applying an electric field to an electrophoresis support having negative surface charges, causes hydration of the zone for loading the sample to be separated when said zone carries a compression mark resulting from loading the sample.

Abstract: The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an electrophoresis support after depositing a solution of lectin onto the electrophoresis support in a predetermined localised zone, under conditions which permit interaction between said lectin and the ALP isoenzymes contained in the analysed biological sample, deposition of the lectin solution further being carried out under conditions which are suitable to allow separation of the ALP isoenzymes constituted by the osseous fraction and by the hepatic fraction.

Abstract: The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an electrophoresis support after depositing a solution of lectin onto the electrophoresis support in a predetermined localized zone, under conditions which permit interaction between said lectin and the ALP isoenzymes contained in the analyzed biological sample, deposition of the lectin solution further being carried out under conditions which are suitable to allow separation of the ALP isoenzymes constituted by the osseous fraction and by the hepatic fraction.

Abstract: The invention relates to a separation procedure for Lp(a) and the various lipoproteins contained in a biological sample by electrophoresis. The present invention is characterized in that the electrophoretic migration of the lipoproteins is carried out under conditions such that the electrophoresis gel and/or the above-mentioned biological sample contains compounds capable of modifying the electrophoretic mobility of Lp(a) differentially with respect to that of the other lipoproteins.