Abstract: An immunogenic agent which comprises a killed strain of Burkholderia pseudomallei, or a combination of components thereof which combination produces a protective immune response in an animal to whom it is administered, and which comprises at least two members selected from the group consisting of (i) a lipopolysaccharide of Burkholderia pseudomallei, (ii) a capsular polysaccharide of Burkholderia pseudomallei and (iii) a protein of Burkholderia pseudomallei or an immunogenic variant thereof or an immunogenic fragment of either of these, or a nucleic acid which expresses said protein, immunogenic variant or immunogenic fragment thereof in a host animal; for use in the prevention or treatment of infection by Burkholderia pseudomallei and/or Burkholderia mallei.

Abstract: A targeted approach is described for the production of biological recognition elements capable of fast, specific detection of anthrax spores on biosensor surfaces. Single chain antibodies (scFvs) are produced to EA1, a Bacillus anthracis S-layer protein that is also present, although is not identical, in related Bacillus species. These antibodies detect Bacillus anthracis EA1 protein and intact spores with a high degree of specificity, but do not detect other Bacillus species. Recombinant anti-EA1 scFvs were isolated from an B. anthracis immune library that contained antibody genes raised against B. anthracis spores and purified exosporium. Two approaches for scFv selection are disclosed; standard (non-competitive) panning, and competitive panning. The non-competitive bio-panning strategy isolated scFvs that recognised EA1 from B. anthracis, but also cross-reacted with other Bacillus species.

Abstract: A Subliminal Image System is described whereby a subliminal image, data set or message is transmitted by simple flashing light sources and captured by a camera system.

Abstract: An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

Abstract: Method and apparatus for carrying out a thermal cycling reaction, wherein a succession of samples is conveyed through a series of sequentially arranged temperature control sites, each of the sites comprising means for supplying an electric current to, or inducing an electric current in, sample-containing vessels passing through it so as to induce temperature changes in the samples. Also provided is a sample support and its production, the support comprising a succession of sample vessels arranged sequentially one behind the next, preferably in the form of a linked chain, the support comprising an electrically conducting, preferably plastics, material which heats when an electric current passes through it.

Abstract: A valve assembly (20) for a respirator comprises a valve body (25) having a valve outlet portion and a valve inlet portion which together define a valve cavity (30) for a valve mechanism (24) permitting gas flow from the valve inlet portion to the valve portion member, an air purge means comprising a purge inlet, connectable to an air pressure supply means, an air purge outlet (27) and an air deflection means (28) in which the deflection means is spatially arranged relative to the valve mechanism and purge outlet so that, in use, air exiting the purge outlet and incident the air deflection means provides a curtain of air over the valve mechanism.

Abstract: Improved apparatus for collecting particles from air comprises a cyclone having air inlet and outlet means, means maintaining an airflow there through and means delivering a collecting fluid from a reservoir to particles separated from the air flow in the cyclone, in which the air inlet means is associated with means for heating the incoming air. The apparatus, which is optionally provided with enclosure means for trapping heat, prevents freezing of the collecting fluid at temperatures near to and even below 0° C.

Abstract: The invention provides use of a polymer composition comprising polyethylenimine and one or both of polyvinyl alcohol and polyvinyl alcohol co-ethylene for protection against harmful and/or noxious agents. The invention further provides a laminate suitable for providing protection against harmful and/or noxious agents, the laminate comprising a layer of a polymer composition comprising polyethylenimine and one or both of polyvinyl alcohol and polyvinyl alcohol co-ethylene is also disclosed.

Abstract: Analytical methods using RNA probes for the detection or analysis of nucleic acid sequences is described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents.

Abstract: A novel single point leaky waveguide structure and its use as an optical sensor for the detection of particles is disclosed. The waveguide structure is fabricated to increase the overlap of the evanescent field extension from the sensor surface with particles in the bulk solution of a flowing system so as to place most of the volume of the particles within the evanescent field. Increasing the overlap of the evanescent field with the particles and permitting mode propagation along the direction of flow for a few millimetres provides an effective interrogation approach for multiple particle detection in a single flow channel.

Abstract: Detail is apparatus for collecting particles from a liquid, including a first electrode having an upper surface defining one or more substantially circular areas of a predetermined diameter separated from a counter electrode, having an uppersurface bounding at least in part the circular area or areas of the first electrode, by a predetermined gap and equipment for applying an alternating potential difference of a predetermined magnitude at a predetermined frequency lower than the charge relaxation frequency of the liquid across the electrodes whereby particles from the liquid are focused onto the circular area or areas of the first elect through an induced electro-osmotic flow in the liquid.

Abstract: An apparatus for characterising an input signal within abroad frequency band by comparing the same input signal in a plurality of channels, in order to operate digital Electronic Support Measures (ESM) which require a broad bandwidth to function. The apparatus comprises one or more signal input bands spread across the broad frequency band, a means of splitting the input signal in each input band into a plurality of separate channels, and a means of sampling each channel, wherein the sampling means in each channel runs at a different clock rate from sampling means in each of the other channels within the input bands, so as to remove the ambiguities inherent in frequency aliasing.

Abstract: A photomultiplier tube circuit with reduced power consumption comprising a photomultiplier tube having a plurality of dynodes, charging circuitry for providing charge to the plurality of dynodes and an oscillator for providing a high voltage supply to the charging circuitry characterised in that the photomultiplier tube circuit further comprises means for sampling the voltage of at least one of the dynodes and a switching means for switching the oscillator on and off with respect to the at least one dynode voltage sampled.

Abstract: An expendable infra-red radiating means having a rupturable container 1 housing a plurality of decoy plates (11) and an ignition means (17) for igniting the decoy plates (11). Each of the decoy plates (11) comprises a composition of a metal and an oxidant capable of an exothermic combustion reaction upon ignition which produces negligible quantities of radiation in the visible or ultra-violet regions and which results, after the combustion reaction is completed, in the decoy plates (11) containing hot metal emitting infra-red radiation.

Abstract: A system for monitoring a live population uses a unit as a local data collection device to derive data on the physical condition of a population member. The unit has a local processor for correlating and storing the physical condition data relative to time and a mechanism for transferring the stored data from the local processor to a remote data collection device. The remote data collection device also receives data relating to the location of the population member relative to time, and analyzes the physical condition and location data for unusual events, providing a signal on the occurrence of an unusual event. The physical condition may be derived from a sensor, which is a non-invasive sensor for measuring pulse rate, temperature and oxygen levels. The location data is provided from a locator incorporated in the unit.

Abstract: Self-calibrating apparatus and methods for dilution of an analyte-containing fluid, and in particular for dilution of analyte concentrations beyond the normal measurement range of a detector, without the requirement for other detectors. The apparatus is preferably a respirator test system for determining respirator mask fit in the presence of high aerosol challenge concentrations.

Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.

Abstract: An aerosol formulation comprising a biodegradable microsphere comprising a non-living reagent, such as a sub-unit vaccine, that produces a protective immune response in a mammal to whom it is administered. Nebulizers and inhalers containing such formulations are also described and claimed.

Abstract: There is disclosed apparatus for directing particles entrained in a fluid, comprising a chamber having a first wall, including means for generating a sound wave having a frequency v, and a second, opposite wall capable of reflecting the sound wave in which the first and second wall define a conduit for the passage of the fluid, and in which the thickness of the second wall is such that the path length of the standing wave in the second wall is a multiple of about ½ the wavelength ?r of the sound wave therein.

Abstract: A photomultiplier tube circuit with reduced power consumption comprising a photomultiplier tube having a plurality of dynodes, charging circuitry for providing charge to the plurality of dynodes and an oscillator for providing a high voltage supply to the charging circuitry characterised in that the photomultiplier tube circuit further comprises means for sampling the voltage of at least one of the dynodes and a switching means for switching the oscillator on and off with respect to the at least one dynode voltage sampled.