Abstract: Novel uracil-DNA glycosylase (UDG) derived from Photobacterium leiognathi and uses thereof are disclosed. The novel UDG is an enzyme isolated from Photobacterium leiognathi, which is a psychrophilic bacterium, and is characterized by having an activity of degrading uracil bases from uracil-containing DNA substrate and being easily denatured at low temperatures. Accordingly, the UDG can be effectively used to eliminate carryover contamination occurring during the nucleic acid amplification reaction, resulting in increased accuracy (elimination of false positives), purity and amplification efficiency in nucleic acid amplification reactions.

Abstract: The present disclosure relates to detection of target nucleic acid by LPHO-assisted PTO cleavage and extension (L-PTOCE) assay. The method and composition according to the present disclosure ensures detection of one or more target nucleic acids with improved accuracy and convenience.

Abstract: A method, particularly a loop-mediated isothermal amplification (LAMP) method for amplifying a target nucleic acid using beta-hydroxy acid is disclosed. The beta-hydroxy acid used in the method can improve the amplification rate of a target nucleic acid to reduce the time required for a LAMP reaction as well as inhibit non-specific amplification to reduce the possibility of false positives.

Abstract: A tagged loop primer and an isothermal nucleic acid amplification method using the tagged loop primer are disclosed. The tagged loop primer contains a single-stranded interactive dual labeling system. The tagged loop primer for a loop-mediated isothermal amplification reaction can be easily prepared, and furthermore, Using the tagged loop enables the effective simultaneous detection of both single target nucleic acids and multiple target nucleic acids.

Abstract: The present invention relates to a method for evaluating the performance of a hot start nucleic acid polymerase, a method for obtaining a quality control-capable (QC-able) hot start nucleic acid polymerase, and a method for providing a blended hot start nucleic acid polymerase that mimics the performance of a target hot-start nucleic acid polymerase. According to the present invention, by using an activity profile of a hot start nucleic acid polymerase for heat activation time, it is possible to evaluate the performance of the hot start nucleic acid polymerase, to provide a QC-able hot start nucleic acid polymerase, and to provide a blended nucleic acid polymerase that mimics the performance of a target hot start nucleic acid polymerase.

Abstract: A method for detecting a target nucleic acid in a sample by Quenching-PTO Cleavage and Extension (Q-PTOCE) assay is disclosed. The method has an advantage in that the signal detection temperature can be selected within a higher temperature range by utilizing not only the cleavage of the Q-PTO and hybridization between the reporter-carrying fragment and the CTO, but also the extension of the reporter-carrying fragment after hybridization.

Abstract: Provided is a tube conveying device for conveying tubes located at preset positions among a plurality of tubes in a storage part in which the plurality of tubes are arranged. The tube conveying device may comprise: a body; a suction structure including suction members that are located on the bottom surface of the body and suction air so as to maintain contact with the upper surface of the tube; and an elastic structure including pusher blocks that protrude from the bottom surface of the body and provide an elastic force against the upper surface of the tube in the direction from the body to the tube, wherein at least one of the pusher blocks is arranged on the bottom surface of the body so as to be located between the suction members.

Abstract: According to the disclosure, a target analyte detection cartridge includes a sample chamber into which sample is inserted, a metering chamber connected to the sample chamber to meter a predetermined amount of sample, a mixing chamber connected to the metering chamber to receive a magnet bead, and a waste chamber connected to the metering chamber. The metering chamber has one side connected to each of the sample chamber and the mixing chamber and another side connected to the waste chamber. A first pneumatic flow path to which pneumatic pressure is provided is connected to a waste flow path that joins the other side of the metering chamber to connect the metering chamber and the waster chamber.

Abstract: An amplification data display method according to the present invention comprises the steps of: displaying a plate display area and an integrated data display area; displaying, in the plate display area, plates where nucleic acid amplification reactions have been performed; and displaying amplification data in the integrated data display area, wherein: two or more plates are displayed in the plate display area; each of the plates includes a plurality of reaction wells; the amplification data are generated from the reaction wells by the nucleic acid amplification reactions; and amplification data for a plurality of reaction wells selected from the reaction wells of two or more of the displayed plates are displayed in the integrated data display area.

Abstract: The present invention relates to a computer-implemented method for providing alignment information of oligonucleotides for a target nucleic acid sequence. The present invention can provide semi-global alignment of oligonucleotides for a target nucleic acid sequence by applying dynamic programming. The present invention can provide alignment information on the entire length of an oligonucleotide more accurately and at a faster rate with less computer resources than conventional methods, and can provide the coverage of an oligonucleotide for a plurality of target nucleic acid sequences.

Abstract: The present invention relates to a method for amplifying at least three target nucleic acid molecules with reduced primer dimer formation in a multiplex amplification reaction. The method of present invention can inhibit primer dimer formation and hence generation of nonspecific amplification products in an effective manner in a multiplex amplification reaction for at least three target nucleic acid molecules.

Abstract: A light detection module according to the present disclosure includes an optical fiber mounting unit having a plurality of optical fibers disposed on one side thereof; a filter wheel spaced apart from the other side of the optical fiber mounting unit, the filter wheel having a plurality of filters; a drive unit for rotating the filter wheel; a light source unit for generating excitation light passing through the filter wheel; and a detection unit for detecting emission light passing through the filter wheel, wherein the plurality of filters includes a plurality of excitation light filters for filtering the excitation light and a plurality of emission light filters for filtering the emission light.

Abstract: The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adoptable for detection of the presence of the target nucleic acid sequence.

Abstract: The present invention relates to a method of detecting n target nucleic acids in a sample, and more particularly, to a method of detecting n target nucleic acids in a sample in a real-time manner in one reaction vessel by being configured such that signals from one or two target nucleic acids are detected at each of n (n is an integer of 3 or more) detection temperatures despite the use of a single fluorescent label.

Abstract: Embodiments of the disclosure relate to a device and method for detecting light in a plurality of independent reaction regions. According to the disclosure, a light detection device includes a plurality of thermally independent reaction regions, a movable optical module for irradiating the reaction regions with lights of a plurality of wavelengths, detectors for detecting lights emitted from the reaction regions, and a controller controlling the reaction regions, the optical module, and the detectors.

Abstract: The present invention provides a method for providing a target nucleic acid sequence data set of a target nucleic acid molecule. The larger number of synonyms are retrieved from reliable sources than that of synonyms extracted by user experience. Thus, the target nucleic acid sequence data group of the target nucleic acid molecule retrieved based on the larger number of synonyms may cover various variant sequences of the target nucleic acid molecule.

Abstract: An apparatus for transferring a tube includes a first conveyor where a tube is transferred, a second conveyor located at a lower level than the first conveyor, and a sliding structure including a guiding body positioned between the first conveyor and the second conveyor and guiding the tube provided in the first conveyor so that the tube is slid down and a controller configured to control so that the tube that is slid down is selectively provided to the second conveyor.

Abstract: An embodiment provides an optical signal detection device including a sample holder configured to accommodate a plurality of samples, a light source module including a plurality of light source units dedicated to each sample area to irradiate light to a plurality of sample areas on the sample holder, a filter module including a plurality of movable moving units for filtering light from the light source unit and a detection module configured to detect emission light of the sample.

Abstract: A device for heating a sample according to the present disclosure includes: a thermal block unit accommodating a reaction vessel; a heat transfer module thermally connected to the thermal block unit; and a heat sink thermally connected to the heat transfer module, wherein the thermal block unit includes: a thermal block having a plurality of accommodating portions for accommodating the reaction vessel; and a heating plate having a plurality of holes into which the plurality of accommodating portions are inserted.

Abstract: The present disclosure relates to a light module comprising a plurality of light sources emitting light to excite samples; a light source wheel accommodating the plurality of light sources; a plurality of filters filtering light emitted by the light sources; a filter wheel accommodating the plurality of filters; and a motor rotating the light source wheel and the filter wheel.