Abstract: A method for isolating proteins from prokaryotes is disclose. The method enables easy break down of bacterial cell walls to obtain intact proteins without damage by a simple process of adding organic solvents including lower alcohols or nitrile derivatives; or applying osmotic stimulation to samples containing pathogenic bacteria or the like. The method may be usefully applied to rapid and accurate identification of periplasmic proteins of gram-negative bacteria without additional purification process.
Type:
Application
Filed:
January 27, 2022
Publication date:
March 14, 2024
Applicant:
SEEGENE MEDICAL FOUNDATION
Inventors:
Jong Kee CHUN, Je Hyun BAEK, Won Suk YANG, Saeyoung LEE, Dong Huey CHEON, Heejung JANG, Seohyun HWANG