Abstract: A unique valve assembly for simulated bed moving chromatography employs a combination of pressure compatible sealable materials that permit minimal frictional interaction between moving parts, ensures alignment of parts, and provides a method for conducting SMB countercurrent chromatography on a small scale, in which purity and separation efficiency are enhanced. Machine designs are disclosed which embody the SMB concepts. Finally, a quick disconnect connector and adaptor is provided for mounting chromatographic columns in an SMB device. Demonstration of this system in a fructose-glucose isomer separation with purity-recovery trade-off for this device is also presented.
Type:
Grant
Filed:
September 26, 2005
Date of Patent:
June 9, 2009
Assignee:
SEMBA Inc.
Inventors:
Anil Rajaram Oroskar, Saurabh Paresh Parikh, Asha Anil Oroskar, Kenneth U. Johnson, Anthony Joseph Escarcega
Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.
Type:
Application
Filed:
August 30, 2007
Publication date:
March 6, 2008
Applicant:
SEMBA, INC.
Inventors:
Joerg Baier, Robert Mierendorf, Anthony Grabski, Andrew Wilke, Anil Oroskar
Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.
Abstract: The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.
Type:
Application
Filed:
August 30, 2007
Publication date:
March 6, 2008
Applicant:
SEMBA, INC.
Inventors:
Robert Mierendorf, Herbert Paaren, Anthony Grabski, Andrew Wilke, Joerg Baier, Anil Oroskar