Patents Assigned to SemBioSys Genetic Inc.
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Patent number: 8597694Abstract: The physical stability of oleosomes is preserved in a composition by introducing a multihydric alcohol and an acid that can reduce the pH of the composition of less than 6. The resultant composition can be useful in the manufacture of cosmetic, food, and pharmaceutical products, among others.Type: GrantFiled: April 10, 2009Date of Patent: December 3, 2013Assignee: Sembiosys Genetics, Inc.Inventors: Jacob Guth, Joseph Boothe, Mitch Beazer, Kent Shafer
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Publication number: 20120255066Abstract: Disclosed are methods for modification of fatty acid composition and/or seed oil content in plants. In particular are methods to over-express acyl-CoA binding proteins (ACBPs) within the cells of developing seeds are provided. Over-expressing ACBPs under the control of a seed preferred promoter increases polyunsaturated fatty acid (PUFA) levels as compared to wild-type controls.Type: ApplicationFiled: March 17, 2008Publication date: October 4, 2012Applicants: THE GOVERNORS OF THE UNIVERSITY OF ALBERTA, SEMBIOSYS GENETICS INC.Inventors: Maurice M. Moloney, Randall Joseph Weselake, Cory L. Nykiforuk, Olga Petrivna Yurchenko
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Patent number: 7786352Abstract: Methods for the production of an apolipoprotein in plants are described. In one embodiment, the present invention provides a method for the expression of apolipoprotein in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5? to 3? direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant cells; and (ii) a nucleic acid sequence encoding an apolipoprotein polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and growing the plant cell into a mature plant capable of setting seed wherein the seed expresses apolipoprotein.Type: GrantFiled: November 15, 2004Date of Patent: August 31, 2010Assignees: Sembiosys Genetics Inc., UTI Limited PartnershipInventors: Maurice M. Moloney, Alexandra Reid, Cory Lee Nykiforuk, Joseph G. Boothe
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Patent number: 7666628Abstract: The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens.Type: GrantFiled: January 26, 2004Date of Patent: February 23, 2010Assignee: SemBioSys Genetics Inc.Inventors: Maurice M. Moloney, Gijs Van Rooijen
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Patent number: 7642346Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.Type: GrantFiled: March 19, 2004Date of Patent: January 5, 2010Assignees: Sembiosys Genetics Inc., Commonwealth Scientific and Industrial Research Organization (CSIRO)Inventors: Sarita Chaudhary, Gijs Van Rooijen, Maurice M. Moloney, Surinder Singh
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Publication number: 20090282583Abstract: Commercial production of human insulin can be effected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.Type: ApplicationFiled: May 12, 2009Publication date: November 12, 2009Applicant: SEMBIOSYS GENETICS INC.Inventors: MAURICE M. MOLONEY, JOSEPH BOOTHE, RICHARD KEON, CORY NYKIFORUK, GIJS VAN ROOIJEN
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Patent number: 7585645Abstract: The present invention provides novel emulsion formulations which comprise oil bodies. The invention also provides a method for preparing the emulsions and the use of the emulsions in a variety of products including food products, personal care products and pharmaceutical products. In a preferred embodiment the emulsions comprise thioredoxin and/or thioredoxin reductase.Type: GrantFiled: July 5, 2001Date of Patent: September 8, 2009Assignee: Sembiosys Genetics Inc.Inventors: Harm M. Deckers, Gijs van Rooijen, Joseph Boothe, Janis Goll, Maurice M. Moloney, Bipin K. Dalmia
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Patent number: 7547821Abstract: Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.Type: GrantFiled: June 17, 2004Date of Patent: June 16, 2009Assignee: SemBioSys Genetics Inc.Inventors: Maurice M. Moloney, Joseph Boothe, Richard Keon, Cory Nykiforuk, Gijs Van Rooijen
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Patent number: 7531325Abstract: An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.Type: GrantFiled: December 19, 2002Date of Patent: May 12, 2009Assignee: SemBioSys Genetics Inc.Inventors: Gijs Van Rooijen, Joenel Alcantara, Maurice M. Moloney
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Patent number: 7501265Abstract: An improved method for recovering recombinantly produced polypeptides is described. The method involves expressing the recombinant polypeptide as a fusion protein with a pro-peptide. The pro-peptide-polypeptide fusion protein can be cleaved and the recombinant polypeptide released under the appropriate conditions.Type: GrantFiled: April 23, 1998Date of Patent: March 10, 2009Assignee: SemBioSys Genetics Inc.Inventors: Maurice Moloney, Joenel Alcantara, Gijs Van Rooijen
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Patent number: 7390936Abstract: A method for the commercial production of chymosin which involves the recombinant expression of chymosin in plant seeds, that enables chymosin to accumulate to at least 0.5% (w/w) of total seed protein is described. An improved method for the isolation of chymosin from the seed is also described.Type: GrantFiled: August 23, 2000Date of Patent: June 24, 2008Assignee: SemBioSys Genetics Inc.Inventors: Gijs Van Rooijen, Keon Richard Glenn, Yin Shen, Joseph Boothe
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Patent number: 7332587Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.Type: GrantFiled: October 1, 2002Date of Patent: February 19, 2008Assignee: SemBioSys Genetics Inc.Inventors: Maurice Moloney, Joseph Boothe, Gijs Van Rooijen
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Publication number: 20070245431Abstract: Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII.Type: ApplicationFiled: March 15, 2007Publication date: October 18, 2007Applicants: MARTEK BIOSCIENCES CORPORATION, SEMBIOSYS GENETICS INC.Inventors: James Metz, Jerry Kuner, James Lippmeier, Maurice Moloney, Cory Nykiforuk
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Publication number: 20070196914Abstract: The present invention provides novel emulsions that comprise oil bodies. The invention also relates to novel methods for generating formulations comprising oil bodies and active ingredients wherein the active ingredient is partitioned into the oil body. The methods are particularly useful for generating emulsions with either hydrophobic or amphipathic biologically active agents.Type: ApplicationFiled: October 4, 2004Publication date: August 23, 2007Applicant: SemBioSys Genetics Inc.Inventors: Elizabeth Murray, Joseph Boothe, Nancy-Ann Markley
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Publication number: 20070006343Abstract: The present invention describes novel polypeptide structures based on oleosin molecules which are capable of being targeted to oil bodies in plants. The modified oleosin polypeptides are obtained by performing modifications in the hydrophobic domain encoding sequence of an oleosin cDNA. The present invention describes methods to obtain such polypeptides in vivo. The novel oleosins may be used to deliver a recombinant (non-oleosin) protein to oil bodies.Type: ApplicationFiled: June 18, 2004Publication date: January 4, 2007Applicant: SEMBIOSYS GENETICS INCInventors: Maurice Moloney, Rodrigo Siloto
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Patent number: 7098383Abstract: Improved methods for the production of multimeric-protein-complexes, such as redox proteins and immunoglobulins, in association with oil bodies are described. The redox protein is enzymatically active when prepared in association with the oil bodies. Also provided are related nucleic acids, proteins, cells, plants, and compositions.Type: GrantFiled: June 21, 2002Date of Patent: August 29, 2006Assignee: SemBioSys Genetics Inc.Inventors: Steven Szarka, Gijs Van Rooijen, Maurice Moloney
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Patent number: 7091401Abstract: The present invention provides a method of preparing epidermal growth factors in plants and transgenic plant seeds containing epidermal growth factors. The method provides an economical way to produce epidermal growth factors.Type: GrantFiled: December 20, 2002Date of Patent: August 15, 2006Assignee: Sembiosys Genetics Inc.Inventors: Maurice M. Moloney, Gijs Van Rooijen
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Patent number: 6924363Abstract: A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.Type: GrantFiled: November 7, 2000Date of Patent: August 2, 2005Assignee: SemBioSys Genetics Inc.Inventors: Maurice Moloney, Joseph Boothe, Gijs van Rooijen
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Publication number: 20040255350Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters.Type: ApplicationFiled: March 19, 2004Publication date: December 16, 2004Applicant: SemBioSys Genetic Inc.Inventors: Sarita Chaudhary, Gijs van Rooijen, Maurice M. Moloney, Surinder Singh
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Patent number: 6777591Abstract: Novel methods for the expression of non-native genes in flax seeds and the seeds of other plant species are provided. The methods involve the use of seed-specific promoters obtained from flax. Additionally provided are novel flax seed-specific promoters, chimeric nucleic acid constructs comprising novel flax seed-specific promoters, transgenic plant cells, transgenic plants and transgenic plant seeds containing novel flax seed-specific promoters. The promoters and methods are useful, for example, for altering the seed oil and protein composition in flax seed or other plant seeds.Type: GrantFiled: August 25, 2000Date of Patent: August 17, 2004Assignees: SemBioSys Genetics Inc., Commonwealth Scientific and Industrial Research Organisation (CSIRO)Inventors: Sarita Chaudhary, Gijs van Rooijen, Maurico Moloney, Surindor Singh