Abstract: A point-of-use analysis with biosensors for peripheral body fluids (e.g., saliva, urine and sweat) and interstitial fluid provides measurements that gain insights to the stress response and inflammation. These measurements may be used in a closed-loop approach in which they are analyzed, and a subject's progress is measured and therapy controlled according to that progress. The point-of-use biosensors provide an opportunity to better understand the effects of stress in real-time and during normal life activities. The digital point-of-use biosensor device enable personalized medicine for many disease conditions.