Abstract: The present invention relates to a exsiccated or lyophilized composition comprising: a nucleic acid polymerization enzyme and cellobiose, in which the enzyme is stable for a period of time, even at a temperature of up to 55° C. The composition of the invention can also comprise further reagents, such as salts, primers specific for a template DNA present in a sample, probes, etc. The invention relates to a method for preparing an exsiccated or lyophilized composition comprising a nucleic acid polymerization enzyme and cellobiose, possibly in containers, in which the enzyme is lyophilized and ready for use in molecular biology applications upon addition of the sample.
Type:
Grant
Filed:
May 19, 2010
Date of Patent:
March 1, 2016
Assignee:
Sentinel CH S.p.A.
Inventors:
Ugo De Luca, Luigi Roveda, Maurizio Gramegna
Abstract: The present invention relates to a exsiccated or lyophilized composition comprising: a nucleic acid polymerization enzyme and cellobiose, in which the enzyme is stable for a period of time, even at a temperature of up to 55° C. The composition of the invention can also comprise further reagents, such as salts, primers specific for a template DNA present in a sample, probes, etc., and possibly other stabilizing compounds. The invention relates to a method for preparing a exsiccated or lyophilized composition comprising a nucleic acid polymerization enzyme and cellobiose, possibly in containers, in which the enzyme is lyophilized and ready for use in molecular biology applications upon addition of the sample.
Type:
Application
Filed:
May 19, 2010
Publication date:
March 15, 2012
Applicant:
Sentinel CH S.p.A.
Inventors:
Ugo De Luca, Luigi Roveda, Maurizio Gramegna