Abstract: The invention relates to a method for the Bio-transformation of Thiocolchicine a colchicinoid compound into its glycosylated form using a selective microorganism Providencia vermicola. Use of bacterial strain, Providencia vermicola for microbial bio-transformation of thiocolchicine (TCN) to their corresponding 3-0-glycosyl derivative as well as specific strains of that species Providencia vermicola for microbial transformation of other colchicinoid compounds such as colchicine, thiocolchicine, 3-demethylcolchicine, 3-demethylthiocolchicine and N-deacetylthiocolchicine to its corresponding glycosylated form and subsequent isolation of the transformed compounds from the bacterial culture medium is disclosed. Providencia vermicola (MTCC 5578) a gram negative bacteria has been identified having trans-glycosylation ability and convert thiocolchicine (TCN) to Thiocolchicocide (TCS).

Abstract: The present invention discloses a stable amorphous form of Rifaximin characterised by having X-ray powder diffraction pattern as given in FIG. 1, having a 2? peaks at 7.2 and having moisture content in the range of 3% to 4% preferably, 3.4% to 3.7%. This invention also discloses a novel process for its preparation.