Abstract: The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wall, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
Type:
Grant
Filed:
November 14, 2019
Date of Patent:
December 14, 2021
Assignees:
SERVICIO GALEGO DE SAÚDE (SERGAS), FUNDACIÓN PROFESOR NOVOA SANTOS
Inventors:
Germán Bou Arévalo, María Clara Póvoa Cabral, Astrid Pérez Gómez, María Merino Carballeira, Alejandro Beceiro Casas, Patricia García Fernández
Abstract: The present invention refers to a method for the production of live attenuated bacterial strains, suitable as vaccine candidates, comprising the steps of: A. providing a bacterial strain capable of expressing glutamate racemase and possibly D-amino acid transaminase and comprising a peptidoglycan cell wail, and B. inactivating the gene or genes encoding for the glutamate racemase enzyme and, if needed, the gene or genes encoding for the enzyme D-amino acid transaminase in such way that the bacterial strain is no longer capable of expressing a functional glutamate racemase and/or a functional D-amino acid transaminase; wherein the inactivation of said genes causes said bacterial strain to be auxotrophic for D-glutamate.
Type:
Grant
Filed:
October 13, 2014
Date of Patent:
December 31, 2019
Assignees:
SERVICIO GALEGO DE SAÚDE (SERGAS), FUNDACIÓN PROFESOR NOVOA SANTOS
Inventors:
Germán Bou Arévalo, María Clara Póvoa Cabral, Astrid Pérez Gómez, María Merino Carballeira, Alejandro Beceiro Casas, Patricia García Fernández
Abstract: Disclosed embodiments include a method for retinal image analysis implemented in a medical system with one or more processors comprising the method steps of: (a) locating an optical disk on the retinal images and establishing a plurality of circumferences centered at the disk; (b) detecting a plurality of vessels within a region defined by said circumferences' radii using a crease-based algorithm; (c) extracting a plurality of vessel segments based on a deformable models (snakes) algorithm; and d) automatically classifying the vessels and computing clinical parameters.
Type:
Grant
Filed:
July 27, 2011
Date of Patent:
January 15, 2013
Assignees:
Universidade da Coruna-Otri, Universidade de Santiago de Compostela, Servicio Galego de Saude (Sergas)
Inventors:
Francisco Gómez-Ulla de Irazazábal, Manuel Francisco González Penedo, Antonio Pose Reino, Noelia Barreira Rodríguez, Marcos Ortega Hortas, María José Carreira Nouche, Antonio Mosquera González
Abstract: Disclosed embodiments include a method for retinal image analysis implemented in a medical system with one or more processors comprising the method steps of: (a) locating an optical disk on the retinal images and establishing a plurality of circumferences centered at the disk; (b) detecting a plurality of vessels within a region defined by said circumferences' radii using a crease-based algorithm; (c) extracting a plurality of vessel segments based on a deformable models (snakes) algorithm; (d) measuring a plurality of vessel calibers; (e) classifying each vessel as a vein and an artery; and (f) computing an arteriolar-to-venular ratio.
Type:
Grant
Filed:
February 1, 2011
Date of Patent:
January 8, 2013
Assignees:
Universidade da Coruna-Otri, Universidade de Santiago de Compostela, Servicio Galego de Saude (Sergas)
Inventors:
Manuel F. González Penedo, Noelia Barreira Rodriguez, Marcos Ortega Hortas, Antonio Mosquera González, Maria Jose Carreira Nouche, Francisco Gomez-Ulla de Irazazabal, Antonio Pose Reino