Abstract: A reagent bottle cleaning device is provided, comprising a base, a cleaning assembly, and a cleaning block. The cleaning assembly comprises a pushing cylinder and a mounting plate. A plurality of mounting grooves are disposed on the mounting plate. Each mounting groove is provided with a cleaning motor. The cleaning motor is provided with a cleaning screw rod. The cleaning block is provided with cleaning grooves corresponding to the cleaning screw rods. Injecting pumps are disposed between the cleaning block and the cleaning assembly. The injecting pumps are connected to the base by lifting cylinders. Full-automatic quick cleaning of reagent bottles can be realized under the effect of a main control chip.