Abstract: An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.
Type:
Grant
Filed:
July 31, 2022
Date of Patent:
May 23, 2023
Assignees:
Tianjin University of Science and Technology, Shandong Lonct Enzymes Co. LTD
Abstract: An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of Bacillus clausii, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.
Type:
Application
Filed:
July 31, 2022
Publication date:
January 19, 2023
Applicants:
Tianjin University of Science and Technology, Shandong Lonct Enzymes Co. LTD
Abstract: Glucoamylase mutant GA3 with improved specific activity and thermal stability, and its gene and application are provided, belonging to the field of gene engineering. The glucoamylase mutant GA3 of the present invention obtained by sequentially mutation at fixed points starting from wild glucoamylase processes both improved thermal stability and catalytic efficiency, and can be applied to the industries of feed, food, medicine and the like.
Type:
Grant
Filed:
February 6, 2021
Date of Patent:
November 29, 2022
Assignee:
Shandong Lonct Enzymes Co. LTD
Inventors:
Xingji Wang, Kefen Wang, Jie Zhang, Xinwei Tong, Wenlong Liu