Abstract: A high-yield bacterial strain for producing acetoin named Bacillus pumilus XH195 has been deposited in a microorganism deposit center in Germany. The deposit number is DSM 16187. The bacteria of the strain are rod-shaped, 1.5 ?m to 3.0 ?m in length, and 0.6 ?m to 0.7 ?m in diameter. The colony color of the bacterial strain is yellow or white. The strain has the typical fatty acid profile of Bacillus and its physiological and biochemical characteristics align with those of Bacillus pumilus. When the bacteria of strain were cultured at 37° C. for 60 hours with shaking, the yield of acetoin could reach 63.0 g/L or 58.1 g/L, when glucose or sucrose was used as the substrate, respectively.

Abstract: This invention provides a high-yield bacterial strain for producing tetramethylpyrazine named Bacillus pumilus RX3-17. The strain has been deposited in China Center for Type Culture Collection on Apr. 19, 2006. The deposit number is CCTCC M 206043. The bacterial strain, isolated from soil, is rod-shaped, 1.5 ?m to 3.0 ?m in length and 0.6 ?m to 0.7 ?m in diameter. The colony color of the strain is milky-white. The strain has typical physiological and biochemical characteristics of Bacillus pumilus. The 16s rDNA sequence of this strain shares a similarity of 99% with other Bacillus pumilus strains. This invention belongs to the domain of biotechnology. The strain can be applied to the production of tetramethylpyrazine with glucose as the substrate, solving the bottleneck of low yield in bacterial tetramethylpyrazine fermentation.