Abstract: A single-cell screening device, a screening assembly, a screening method, and use thereof. The single-cell screening device includes a body, a plurality of spray holes, heating portions, and a flow channel. The spray holes are located in the body, the heating portions are located in the spray holes and used for heating a liquid entering the spray holes to generate bubbles so as to spray out cells. The flow channel is located in the body and extends to the spray holes according to a preset path, so as to deliver a cell suspension into the spray holes. The single-cell screening device of the present disclosure has a simple design and is easy to fabricate. Cell printing (spraying) using the screening assembly of the present disclosure has a much higher cell printing speed than conventional methods, allowing for a rapid cell sorting process.

Abstract: Provided are a cell screening device and a cell screening method, for use in performing screening on different cells. The cell screening device is provided with: at least two flowing channels, used for allowing a solution containing cells to flow through; a communicating path, used for allowing the adjacent flowing channels to communicate with each other; a detection unit, used for detecting the types of cells in the solution flowing in the flowing channels; and a screening actuator, generating push force according to the detection result of the detection unit, so as to push cells in the solution flowing in the flowing channel to flow into the adjacent flowing channel through the communicating path.