Abstract: The present disclosure relates to a primer and a kit for rapid detection of bacteria and an application thereof, belonging to the technical field of bacterial detection. The present disclosure provides a primer for rapid detection of bacteria, a nucleotide sequence of a forward primer for the detection is shown in SEQ ID NO.1, and a nucleotide sequence of a reverse primer is shown in SEQ ID NO.2; a degenerate base W=A/T in the forward primer, and a degenerate base R=A/G in the reverse primer. The primer of the present disclosure may achieve rapid, wide, high-sensitivity and quantitative detection of bacteria, can be applied in the field of bacterial detection, and is more suitable for the clinical research and application of cells and stem cell-based products.