Patents Assigned to Shanghai Mendel DNA Center Co., Ltd.
  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Patent number: 6818431
    Abstract: The invention relates to genetic modification of DNA polymerase to reduce innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated fluorescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: November 16, 2004
    Assignee: Shanghai Mendel DNA Center Co. Ltd.
    Inventors: GuoFan Hong, Wei-hua Huang
  • DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
    Patent number: 6485909
    Abstract: The invention relates to genetical modification of DNA polymerase to reduce its innate selective sequence-related discrimination against incorporation of fluorescent dye-labeled ddCTP and ddATP in the enzymatic reaction for preparation of samples for automated florescent dye-labeled terminator DNA sequencing. The modified DNA polymerases are more resistant to heat inactivation and are more effective in dideoxynucleotide incorporation than current DNA polymerases.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: November 26, 2002
    Assignee: Shanghai Mendel DNA Center Co., Ltd.
    Inventors: GuoFan Hong, Wei-hua Huang