Abstract: The invention discloses a plant thermo-sensitive male sterile gene mutant tms15 and uses thereof. The nucleotide sequence of the plant thermo-sensitive male sterile gene mutant tms15 is SEQ ID No. 1. The inventor screened the thermo sensitive genic male sterile mutant tms15 by EMS mutagenesis of Zhonghua 11, which showed a male sterile phenotype under high temperature conditions (>28° C.) and restored fertility under low temperature conditions (22˜24° C.). The cytological results shows that the sporogenous cells in tms15 plants proliferated excessively and the tapetum cells are also vacuolized abnormally under high temperature. The normal proliferation of sporogenous cells and the normal development of tapetal cells are restored to some extent after low temperature treatment.

Abstract: The present disclosure provides a functional beverage of dark tea with zero energy for reducing weight and lipid and a preparation method thereof, and relates to the technical field of processing health-promoting beverage. The functional beverage includes 1.5-3.5 parts of dark tea, 0.3-0.7 parts of mulberry leaf, 0.1-0.5 parts of lotus leaf, 0.1-0.3 parts of stevia leaf, and 95.4-97.4 parts of purified water. The preparation process thereof includes treatment of raw materials, circulatory microwave extraction, static settlement, membrane ultrafiltration, and ultra-high temperature instantaneous sterilization (UHT). Compared with the prior art, the functional beverage uses purely natural herb plants without any additives. The obtained product has zero sugar while has the effects of quenching thirst and reducing weight and lipid. Key technologies such as integrated formula, circulatory microwave extraction, and membrane ultrafiltration enable the best flavor and efficacy of the beverage.