Abstract: Disclosed are a gene panel for molecular subtyping and assessing the survival risk of colorectal cancer, and use of agents for detecting the expression levels of the genes in the gene panel in the manufacture of a product, the product being used for determining the molecular subtype of colorectal cancer and assessing the survival risk of a patient with colorectal cancer; the product comprises a Next-Generation Sequencing (NGS) detection kit, a fluorescence quantitative PCR detection kit, a gene chip, and a protein chip. Also disclosed is a method for using the detection kit to molecular subtype and assess the survival risk of colorectal cancer.