Abstract: The present application relates to a method of amplifying genomic DNA of a cell, comprising: (a) providing a reaction mixture, wherein the reaction mixture comprises the genomic DNA, a first type of primer, a second type of primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the first type of primer comprises, in a 5? to 3? orientation, a common sequence and a variable sequence, wherein the common sequence consists of three or two types of bases selected from the group consisting of four types of bases: G, A, C and T, providing that the common sequence does not comprise G and C at the same time, and wherein the second type of primer comprises the common sequence but not the variable sequence; (b) placing the reaction mixture in a first thermal cycle program such that the variable sequence of the first type of primer can pair with the genomic DNA and amplify the genomic DNA to obtain a genomic amplification product, wherein the genomic amplification product comprises the common seq
Type:
Grant
Filed:
August 29, 2016
Date of Patent:
June 22, 2021
Assignee:
SHANGHAI XUKANG MEDICAL SCIENCE & TECHNOLOGY CO., LTD
Abstract: The present application relates to a method of amplifying genomic DNA of a cell, comprising: (a) providing a reaction mixture, wherein the reaction mixture comprises the genomic DNA, a first type of primer, a second type of primer, a mixture of nucleotide monomers, and a nucleic acid polymerase, wherein the first type of primer comprises, in a 5? to 3? orientation, a common sequence and a variable sequence, wherein the common sequence consists of three or two types of bases selected from the group consisting of four types of bases: G, A, C and T, providing that the common sequence does not comprise G and C at the same time, and wherein the second type of primer comprises the common sequence but not the variable sequence; (b) placing the reaction mixture in a first thermal cycle program such that the variable sequence of the first type of primer can pair with the genomic DNA and amplify the genomic DNA to obtain a genomic amplification product, wherein the genomic amplification product comprises the common seq
Type:
Application
Filed:
August 29, 2016
Publication date:
September 6, 2018
Applicant:
SHANGHAI XUKANG MEDICAL SCIENCE & TECHNOLOGY CO., LTD