Abstract: The present invention provides a novel fusion protein suitable for high expression and the production method thereof. The structure of the fusion protein is A-C1-K-(B-C2-K)n-D, wherein A is the upstream peptide; each of C1 and C2 is independently short peptides having 20-40 amino acids in length and containing no Lys in sequence; K is Lys; B is a linker; D is a downstream peptide; n is an integer of 3-30. The fusion protein is expressed with high efficiency and stability in host cells. The purification and enzymatic cleavage technology of the expression product is simple, which is able to produce a short peptide with high efficiency, and has very high value of industrialization.
Type:
Grant
Filed:
June 3, 2003
Date of Patent:
September 14, 2010
Assignees:
Shanghai Centre of Research & Development of New Drugs, Shanghai Newsummit Biopharma Co., Ltd, Shanghai Yizhong Biotechnology Co., Ltd
Abstract: The present invention provides a novel fusion protein suitable to be expressed high effectively and the production method thereof. The structure of the fusion protein is A-C1-K-(B-C2-K)n-D, wherein A is the upstream peptide; each of C1 and C2 is independently short peptides having 20-40 amino acids in length and containing no Lys in sequence; K is Lys; B is a linker; D is a downstream peptide; n is an integer of 3-30. The fusion protein is able to express high effectively and stably in host cells. The purification and enzymatic cleavage technology of the expression product is simple, which is able to produce a short peptide high effectively, and has very high value of industrialization.
Type:
Application
Filed:
June 3, 2003
Publication date:
February 21, 2008
Applicants:
Shanghai Centre of Research & Development of New Drugs, Shanghai Newsummit Biopharma Co., Ltd., Shanghai Yizhong Biotechnology Co., Ltd.