Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
Type:
Application
Filed:
November 18, 2022
Publication date:
May 25, 2023
Applicants:
Oxford Nanopore Technologies PLC, Sharp Life Science EU (Ltd)
Inventors:
Matthew Holden, James White, Andrew John Heron, James Anthony Clarke, Jason Robert Hyde, Benjamin James Hadwen, Sally Anderson
Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
Type:
Grant
Filed:
December 21, 2018
Date of Patent:
December 27, 2022
Assignees:
Oxford Nanopore Technologies PLC, Sharp Life Science EU (Ltd)
Inventors:
Matthew Holden, James White, Andrew John Heron, James Anthony Clarke, Jason Robert Hyde, Benjamin James Hadwen, Sally Anderson
Abstract: Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.
Type:
Application
Filed:
December 21, 2018
Publication date:
January 14, 2021
Applicants:
Oxford Nanopore Technologies Ltd., Sharp Life Science (EU) Ltd
Inventors:
Matthew HOLDEN, James WHITE, Andrew John HERON, James Anthony CLARKE, Jason Robert HYDE, Benjamin James HADWEN, Sally ANDERSON