Abstract: The invention relates to an improved method for agrobacterium transformation and regeneration of plants. The inventive method is characterized in that it consists in sequentially preparing, inoculating and co-cultivating explants. The preparation of each subsequent lot of plants is carried out after a time interval for transforming plant cells and forming an induced resistance with respect to abiotic and biotic stresses in a leaf discs, thereby making it possible to reduce a necrosis degree and the number of somaclonal variations of the transgenic plants.
Type:
Grant
Filed:
October 15, 2003
Date of Patent:
May 24, 2011
Assignee:
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences
Inventors:
Konstantin Aleksandrovich Shestibratov, Sergey Vladimirovich Dolgov
Abstract: The invention relates to an improved method for agrobacterium transformation and regeneration of plants. The inventive method is characterized in that it consists in sequentially preparing, inoculating and co-cultivating explants. The preparation of each subsequent lot of plants is carried out after a time interval for transforming plant cells and forming an induced resistance with respect to abiotic and biotic stresses in a leaf discs, thereby making it possible to reduce a necrosis degree and the number of somaclonal variations of the transgenic plants.
Type:
Application
Filed:
October 15, 2003
Publication date:
May 31, 2007
Applicant:
Shemyakin And Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences