Abstract: An ultraviolet fluorescent barcode reading method and device, the reading method comprising the following steps: S1, irradiating an invisible ultraviolet fluorescent barcode via an ultraviolet light source and activating the invisible ultraviolet fluorescent barcode to be visible; S2, acquiring the image of the barcode activated to be visible; S3, filtering and decoding the barcode image to obtain corresponding barcode information; S4, outputting the barcode information. The reading method reads the invisible ultraviolet fluorescent barcode via a color filter, thus improving the convenience for reading.