Abstract: A gene sequencing method includes: hybridizing a sequencing primer and a molecule to be detected to form a template strand and a primer strand; linking a first nucleotide analog to the primer strand, wherein the first nucleotide analog has a blocking group; performing base pairing on a second nucleotide analog and the nucleic acid molecule to be detected, the second nucleotide analog forming a complex with the nucleic acid molecule to be detected and the first nucleotide analog under the action of metal ions and a polymerase, wherein the second nucleotide analog has a marker; detecting the marker, and identifying a base; and removing the blocking group and the second nucleotide analog, and performing a next cycle of sequencing. The gene sequencing method can completely remove blocking groups and markers without leaving synthetic scars, so that the sequencing length can be increased and the sequencing cost can be reduced.

Abstract: The embodiments of the application provide a base-calling method and device, electronic equipment and a storage medium, and belongs to the technical field of recognition. The base-calling method comprises: acquiring original light intensity data of original base channels; performing crosstalk correction on the original light intensity data to obtain first corrected light intensity data; performing quantile normalization on the first corrected light intensity data to obtain initial light intensity data; performing phase correction on the initial light intensity data to obtain second corrected light intensity data; performing mean normalization on the second corrected light intensity data to obtain target light intensity data; performing intensity comparison and screening on the original base channels according to the target light intensity data to obtain a target base; and splicing multiple target bases to obtain a target base sequence. The embodiments of the application can improve the base-calling accuracy.