Abstract: A rapid detection system for water and impurity of machine-harvested seed cotton in purchase link, including: a seed cotton large-impurity-cleaning device, a seed cotton sample case, a driving and transmission device, a cotton pressing device, an image collection and processing device, and a moisture regain rate detection device and a control cabinet. The seed cotton sample case consists of a positioning plate and a high light transmittance glass case, the weighing device consists of a weighing sensor, a pallet, and an electric push rod. The driving and transmission device consists of the rollers, the motor of conveyor belts and conveyor belts, the cotton pressing device consists of an electric push rod, a connecting rod, a connecting plate, and a high light transmittance glass plate. Said rapid detection system could realize the rapid detection to the impurity rate and moisture regain rate of the seed cotton.

Abstract: An intelligent analysis system applied to ethology of various kinds of high-density minimal polypides is provided, including an operating platform subsystem and an analysis subsystem; the operating platform subsystem is used for providing an activity medium capable of obtaining behaviors of the minimal polypides and shooting the behaviors of the minimal polypides; the behavior collecting module in the analysis subsystem is used for obtaining an ethology video of the high-density minimal polypides through the operating platform subsystem; the analysis modeling module is used for obtaining ethology big data of various kinds of high-density minimal insects according to the ethology video, and establishing an ethology model; the simulation predicting module is used for dynamically predicting comprehensive control strategies and control effects of minimal pests in a field ecosystem according to the ethology model; the visual demonstrating module is used for displaying the above behavior data.

Abstract: Disclosed are a low-cost high-efficiency drip irrigation system for a cotton field and a use method thereof. The drip irrigation system for a cotton field includes a filtering device and a water supply device; wherein the filtering device adopts different types of single filtering devices or combined filtering devices according to different water source types; the water supply device includes multiple stages of main pipes, submain pipes and laterals; the laterals (a drip irrigation tape with labyrinth on one side or a drip irrigation tape inlaid with emitters inside) are laid on the surface of the ground and located below a mulch film. The drip irrigation system adopts the design of large flow (1.5-3.4 L/h), slightly larger pipe diameter (>75 mm), low water pressure (operating pressure of drip irrigation tape being 0.03-0.07 MPa) and suitable drip irrigation uniformity (80%-90%).

Abstract: Provided are catechol nanoparticles, catechol protein nanoparticles, and a preparation method and use thereof. The method includes: adding a tannin compound-containing natural herb medicine into water to obtain a mixture, and subjecting the mixture to heating reflux extraction to obtain a herb medicine extract and subjecting the herb medicine extract to fractionation to obtain the catechol nanoparticles.

Abstract: The application belongs to the technical field of animal or human antibodies, and discloses a TIM-3 nanobody, a preparation method thereof and use thereof. The nanobody is a TIM-3 nanobody with sequence of SEQ ID NO:1. TIM-3 antigen is a transient transfection expression by mammalian cells. TIM-3 antigen is used to screen the nanobody library repeatedly, specific phage of nanobody is obtained, and the target fragment is conducted sequencing. The application uses the HEK293 cell line to express antigen. Using the mammalian expression system to express human protein may maximumly guarantee the original structure of the protein, guarantee the protein to have a post-translational modification and specific modifications of eukaryotic proteins such as glycosylation, which makes the obtained protein have high activity. This method maximizes the original structure and activity of the protein; the nanobodies screened by the application can efficiently and specifically bind to the target.

Abstract: A nanobody and its application based on SARS-CoV-2 S protein are provided, and the present disclosure relates to biomedical technology. The present disclosure chooses the Spike S1+S2 ECD of SARS-CoV-2 as a target, and screens the nanobody against of SARS-CoV-2 by using a nanobody library. After an ELISA test, the Spike S1+S2 ECD target of SARS-CoV-2 can be specifically identified while a SPIKE RBD target is identified, and a binding signal is relatively strong. The corresponding nanobody sequence is constructed into a prokaryotic expression vector for expression and purification to express the target nanobody successfully. After the purification, the purity is greater than 90%. The ELISA test of VHH nanobody showed that the purified nanobody has higher affinity to the two targets.

Abstract: A continuous feeding device for predatory mites, including a feeding part and an isolation part to solve problems existing in the prior art of accurately controlling a feeding quantity of predatory mites, and reducing a number of the feeding devices required for feeding predatory mites, thereby reducing a feeding cost of predatory mites.

Abstract: A nanobody and its application based on SARS-CoV-2 S protein S1 subunit are provided, and the present disclosure relates to biomedical technology. The present disclosure chooses the Spike RBD of SARS-CoV-2 as a target, and screens the nanobody targeting SARS-CoV-2 by using a nanobody library. After an ELISA test, the Spike RBD target of SARS-CoV-2 can be specifically identification while a SPIKE S1+S2 ECD target is identification, and a binding signal is relatively strong. The corresponding nanobody sequence is constructed into a prokaryotic expression vector for expression and purification to express the target nanobody successfully. After purification, the purity is greater than 90%. The ELISA test of VHH nanobody showed that the purified nanobody has higher affinity to the two targets.

Abstract: Disclosed is preparation of a cocktail as a therapeutic agent for cow mastitis and use thereof. A compatibility of phages against the main pathogenic bacteria of cow mastitis is utilized in combination with a specific preparation method to prepare and obtain a therapeutic cocktail preparation. The therapeutic phage cocktail preparation for cow mastitis provided in the disclosure is easy to prepare, short in course of treatment and quick in effect. It has better therapeutic effect on both clinical cow mastitis and recessive mastitis than that of antibiotics and antimicrobial peptide and is expected to solve the problem of bacterial resistance in the treatment of cow mastitis, thereby reducing or getting rid of the troubles of diseases such as mastitis in cows, which is of potential development value in cow farming.

Abstract: The disclosure relates to a co-current co-precipitation method of CoNiO2 thermistor powders. The method comprises the steps of mixing, stirring, precipitating, aging, suction filtration, washing and drying firstly using nickel nitrate and cobalt nitrate as raw materials to obtain cobalt hydroxide, and then calcining in a tubular furnace at an inert atmosphere to prepare CoNiO2 nano powders. The method has the advantages of simple operation, low cost, short cycle, high yield and no environmental pollution, and further oxidization of the CoNiO2 nano material into NiCo2O4 thermistor powders can be effectively avoided through selection and adjustment of calcination process parameters and inert atmosphere. A high-precision, fast-response and small-volume temperature sensor material can be prepared from CoNiO2 thermistor powders obtained by the method of the disclosure.

Abstract: The application belongs to the technical field of animal or human antibodies, and discloses a TIM-3 nanobody, a preparation method thereof and use thereof. The nanobody is a TIM-3 nanobody with sequence of SEQ ID NO: 1. TIM-3 antigen is a transient transfection expression by mammalian cells. TIM-3 antigen is used to screen the nanobody library repeatedly, specific phage of nanobody is obtained, and the target fragment is conducted sequencing. The application uses the HEK293 cell line to express antigen. Using the mammalian expression system to express human protein may maximumlly guarantee the original structure of the protein, guarantee the protein to have a post-translational modification and specific modifications of eukaryotic proteins such as glycosylation, which makes the obtained protein have high activity. This method maximizes the original structure and activity of the protein; the nanobodies screened by the applicaiton can efficiently and specifically bind to the target.