Abstract: The invention was developed in order to effectively crush and disperse various samples easily and efficiently without using any expensive device. By laminating a polyethylene film 3, a net member 7, a net member 9 and a polyethylene film 5 in sequence and welding these members to one another at weld portions 13a and 13b, a bag member 15 is formed. Further by forming a fastener 17 in the vicinity of an opening 15a, a crushing and dispersing bag 1 is completed. After a sample and a dispersing medium are introduced through the opening 15a, the bag is sealed with the fastener 17. Subsequently, when the crushing and dispersing bag 1 is squeezed, the sample is ground by the file-like irregularities formed by the net members 7 and 9 on the inner surface of the bag 1.

Abstract: The object of the present invention is to permeate sample solution uniformly into an absorbent fibrous sheet. The gelation agent soluble in cold water absorbs moisture and forms a gel, thereby forming a gel medium. Microorganisms are thus cultivated. The surface of a waterproof plate 2 is coated with the mixture 3 of the gelation agent soluble in cold water and microorganism culture medium. Further, an absorbent fibrous sheet 4 is laid on the mixture 3. When the sample solution including microorganisms is poured onto the absorbent sheet 4, the solution is dispersed uniformly over the entire of the absorbent fibrous sheet 4 by the phenomenon of capillary attraction resulting from overlapped fibers. Subsequently, the mixture 3 of the gelation agent and culture medium coated on the plate 2 absorbs moisture and forms a gel. The fibers of the absorbent fibrous sheet 4 are buried and adhered into the mixture 3, thereby forming a gel medium for the culture of microorganisms.