Abstract: The present invention relates to DNA segments isolated from Sphingomonas sp. and involved in the biosynthetic production of sphingan polysaccharides to increase the production of the polysaccharide in engineered microorganisms. The present invention also relates to methods of engineering strains of Sphingomonas to produce bacteria which are hyperproducers of sphingan, methods of identifying and utilizing DNA fragments useful to enhance production of sphingan in bacteria and the hyperproducer bacteria.
Type:
Grant
Filed:
January 24, 1996
Date of Patent:
December 29, 1998
Assignees:
Shin-Etsu Cio, Inc., Shin-Etsu Chemical Co., Ltd.
Inventors:
Thomas J. Pollock, Motohide Yamazaki, Linda Thorne, Marcia Mikolajczak, Richard W. Armentrout