Abstract: The present invention discloses a multi-target recombination gene and the application of its protein to prevent and cure Helicobacter pylori. It includes a multi-target fusion polypeptide having amino acid sequence shown in SEQ ID NO:2, a multi-target recombination gene of encoding multi-target fusion polypeptide having nucleotide sequence shown in SEQ ID NO:1, and the application of the multi-target recombination gene or the multi-target fusion polypeptide or specific antibody of the multi-target fusion polypeptide as biological products for preventing and curing Helicobacter pylori. The present invention effectively combines the key target protein UreI for Hp gastric colonization, and antigen target of UreB to create the best drug target.