Patents Assigned to Sigma-Aldrich Co.
  • Patent number: 11896953
    Abstract: Polymeric adsorbents useful for solid phase extraction (SPE) comprising a copolymer formed by copolymerizing at least one hydrophobic monomer including divinylbenzene and at least one hydrophilic monomer including acrylonitrile are provided. Also provided are SPE cartridges including a divinylbenzene-acrylonitrile hydrophilic-lipophilic balance adsorbent. Further provided are methods of using a divinylbenzene-acrylonitrile hydrophilic-lipophilic balance adsorbent in conventional and simplified SPE techniques.
    Type: Grant
    Filed: August 24, 2018
    Date of Patent: February 13, 2024
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: William Betz, Curtis Frantz, Michael Keeler, Michael Ye, Ken Espenschied, Sara Smith, Brittany A. Smith
  • Patent number: 11891510
    Abstract: Described herein are poloxamer compositions for use as a shear protectant in cell culture and methods for preparing and using such compositions.
    Type: Grant
    Filed: December 6, 2018
    Date of Patent: February 6, 2024
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Scott Wilson, Kevin Kent, Chandana Sharma
  • Patent number: 11806693
    Abstract: Methods for producing porous graphic carbon microspheres having improved separation properties over conventional porous graphitic carbons. The methods include dispersing a monovinyl aromatic monomer, a polyvinyl aromatic monomer, and an initiator in a solvent, contacting porous silica microspheres with the monomer dispersion for a time sufficient for the monomers to coat the porous silica microspheres, polymerizing the monomers to form copolymer coated microspheres, sulfonating the copolymer, pyrolyzing the sulfonated copolymer, digesting the carbon microspheres to dissolve the silica leaving porous carbon microspheres, pyrolyzing the porous carbon microspheres, and graphitizing the porous carbon microspheres to form porous graphitic carbon microspheres. Further provided are improved porous graphitic carbon microspheres and chromatography columns including the improved porous graphitic carbon microspheres described herein.
    Type: Grant
    Filed: June 16, 2021
    Date of Patent: November 7, 2023
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: William R. Betz, Michael J. Keeler, Curtis W. Frantz, Jay M. Jones, William Maule, Kenneth G. Espenschied, Cory E. Muraco, Clinton Corman, Maochun Ye
  • Patent number: 11410752
    Abstract: A system, software application and method that allows a customer to protect their proprietary database of compounds and substances while utilizing a retrosynthesis software application is disclosed. The customer's proprietary database is encrypted prior to being provided to the retrosynthesis system. This encrypted is performed using a hash and optionally a salt. The retrosynthesis algorithm then creates synthons as is traditionally done. However, after their creation, the synthons are hashed so that they may be compared to the entries in the customer's proprietary database. In this way, the actual contents of the customer's database are never made available to the retrosynthesis system or software application.
    Type: Grant
    Filed: July 30, 2019
    Date of Patent: August 9, 2022
    Assignees: EMD Millipore Corporation, Sigma-Aldrich Co., LLC
    Inventors: Sarah Trice, Tim Knehans, Maciej Wojcikowski
  • Patent number: 11390913
    Abstract: Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: July 19, 2022
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Brian W. Ward, Carol A. Kreader, Jaime K. Robert, Kenneth E. Heuermann
  • Patent number: 11268144
    Abstract: The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: March 8, 2022
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Gregory D. Davis, Vikas B. Palhan, Carol A. Kreader
  • Patent number: 10988802
    Abstract: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5? phosphate, a 3? with an —H in place of the —OH, and/or a 3? extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for amplification using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: April 27, 2021
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Brian Ward
  • Patent number: 10947517
    Abstract: Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.
    Type: Grant
    Filed: February 13, 2020
    Date of Patent: March 16, 2021
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Fuqiang Chen
  • Patent number: 10928366
    Abstract: A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis.
    Type: Grant
    Filed: January 24, 2008
    Date of Patent: February 23, 2021
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Craig Aurand, Charles Mi, Paul Ross, An Trinh, Hillel Brandes, Michael Ye
  • Patent number: 10767193
    Abstract: Engineered Cas9 systems that utilize alternate protospacer adjacent motifs for target DNA binding, nucleic acids encoding the engineered Cas9 systems, and methods of using the engineered Cas9 systems for modifying target chromosomal sequences in eukaryotic cells.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: September 8, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Timothy Seebeck, Fuqiang Chen, Gregory D. Davis
  • Patent number: 10745716
    Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: August 18, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Fuqiang Chen, Gregory D. Davis
  • Patent number: 10731181
    Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: August 4, 2020
    Assignee: Sigma, Aldrich Co. LLC
    Inventors: Fuqiang Chen, Gregory D. Davis
  • Patent number: 10711266
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: July 14, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol A. Kreader
  • Patent number: 10711296
    Abstract: Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3? sequence having complementarity to a portion of the RNA molecule, a non-complementary 5? sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: July 14, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Kenneth B Heuermann, Carol A Kreader, Jaime K Robert, Brian W Ward
  • Patent number: D962258
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: August 30, 2022
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener
  • Patent number: D962259
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: August 30, 2022
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener
  • Patent number: D962260
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: August 30, 2022
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener
  • Patent number: D962282
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: August 30, 2022
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener
  • Patent number: D970517
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: November 22, 2022
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener
  • Patent number: D980235
    Type: Grant
    Filed: November 12, 2020
    Date of Patent: March 7, 2023
    Assignees: Sigma-Aldrich Co. LLC, EMD Millipore Corporation
    Inventors: Sarah Trice, Lindsey Rickershauser, Xiang Wu, Churl Oh, Nadia Brugger, Emma Gardener