Abstract: Engineered Cas9 systems are disclosed herein.

Abstract: Polymeric adsorbents useful for solid phase extraction (SPE) comprising a copolymer formed by copolymerizing at least one hydrophobic monomer including divinylbenzene and at least one hydrophilic monomer including acrylonitrile are provided. Also provided are SPE cartridges including a divinylbenzene-acrylonitrile hydrophilic-lipophilic balance adsorbent. Further provided are methods of using a divinylbenzene-acrylonitrile hydrophilic-lipophilic balance adsorbent in conventional and simplified SPE techniques.

Abstract: Described herein are poloxamer compositions for use as a shear protectant in cell culture and methods for preparing and using such compositions.

Abstract: Methods for producing porous graphic carbon microspheres having improved separation properties over conventional porous graphitic carbons. The methods include dispersing a monovinyl aromatic monomer, a polyvinyl aromatic monomer, and an initiator in a solvent, contacting porous silica microspheres with the monomer dispersion for a time sufficient for the monomers to coat the porous silica microspheres, polymerizing the monomers to form copolymer coated microspheres, sulfonating the copolymer, pyrolyzing the sulfonated copolymer, digesting the carbon microspheres to dissolve the silica leaving porous carbon microspheres, pyrolyzing the porous carbon microspheres, and graphitizing the porous carbon microspheres to form porous graphitic carbon microspheres. Further provided are improved porous graphitic carbon microspheres and chromatography columns including the improved porous graphitic carbon microspheres described herein.

Abstract: A system, software application and method that allows a customer to protect their proprietary database of compounds and substances while utilizing a retrosynthesis software application is disclosed. The customer's proprietary database is encrypted prior to being provided to the retrosynthesis system. This encrypted is performed using a hash and optionally a salt. The retrosynthesis algorithm then creates synthons as is traditionally done. However, after their creation, the synthons are hashed so that they may be compared to the entries in the customer's proprietary database. In this way, the actual contents of the customer's database are never made available to the retrosynthesis system or software application.

Abstract: Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.

Abstract: The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.

Abstract: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5? phosphate, a 3? with an —H in place of the —OH, and/or a 3? extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for amplification using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.

Abstract: Engineered Cas9 systems are disclosed herein. For example, Cas9-marker fusion proteins are provided. Peptide linkers which facilitate fusion of heterologous proteins to CRISPR proteins in ways that preserve CRISPR functionality are also provided.

Abstract: A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis.

Abstract: Engineered Cas9 systems that utilize alternate protospacer adjacent motifs for target DNA binding, nucleic acids encoding the engineered Cas9 systems, and methods of using the engineered Cas9 systems for modifying target chromosomal sequences in eukaryotic cells.

Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.