Abstract: Polymorphs of chemical compounds that modulate kinase activity, including PI3K activity, and chemical compounds, pharmaceutical compositions, and methods of treatment of diseases and conditions associated with kinase activity, including PI3K activity, are described herein.

Abstract: The present invention provides methods modified oligonucleotides and methods of using the modified oligonucleotides for silencing nucleic acids, wherein the nonspecific effects of nucleic acid silencing are reduced.

Abstract: The present invention provides methods for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography.

Abstract: The present invention provides fluorescent dye compounds and methods of using the compounds for the staining of nucleic acids. In particular, the dye compounds comprise heterocyclic molecules with hydroxy alkyl and aromatic substituents, and the dye compounds form highly fluorescent complexes upon nucleic acid binding.

Abstract: A cap and bottle device is provided that includes a cap, a septum, and a container that individually and collectively improves the capacity of the assembly to securely contain substances. The cap includes internal threads that mate external threads of the container containing the substance. The cap further includes flexible teeth for engaging rigid lugs that are molded into the finish on the neck of the container. The septum sealably fits between the cap and within an opening of the container to provide barrier between the contained substance and the surrounding environment.

Abstract: The present invention provides a plurality of oligonucleotides comprising a semi-random sequence, wherein the semi-random sequence comprises degenerate nucleotides that are substantially non-complementary. Also provided are methods for using the plurality of oligonucleotides to amplify a population of target nucleic acids.

Abstract: The present invention provides an array system comprising a plurality of immobilized oligonucleotides comprising artificial sequences and a plurality of complementary ligation templates, as well as methods and kits for using the array system to analyze populations of nucleic acids. In particular, target nucleic acids are ligated to the immobilized oligonucleotides on the array in the presence of the complementary ligation templates.

Abstract: A consolidated vacuum sublimation module (VSM) can comprise an upper body portion, a middle body portion, a lower body portion, a bushing, and a seal. A vapor director cartridge can be loaded with a crude material and manually inserted into a cartridge-holding member of the lower body portion. Assembly of the middle and upper body portions form a structural unit that can compressively and sealably connect to the lower body portion. During sublimation, heat is effectively transferred to the lower body portion that contains the crude material. A substantial fraction of the middle body portion is conically tapered about a central longitudinal axis to promote desublimation. The middle body portion is a unitary structure that consolidates valving, cooling, and desublimation functions.

Abstract: A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis.

Abstract: The present invention provides compositions, methods, and kits for protecting thermostable DNA polymerase during amplification reactions conducted at a temperature ranging from about 40° C. to greater than 100° C. The composition comprises a thermostable DNA polymerase and an anionic detergent or zwitterionic detergent.

Abstract: The present invention provides a plurality of molecular markers and methods of using the markers for determining whether a cancer will be responsive to a taxane. Also provided are kits comprising the plurality of molecular markers.

Abstract: The present invention provides lysis reagents, containers, methods and kits relating to the extraction or the extraction and isolation of a cellular component from a host cell. More specifically, the invention provides combinations of zwitterionic compounds that may be employed to aide in the extraction or the extraction and isolation of a cellular component from a host cell.

Abstract: The present invention discloses novel methods for the synthesis of oligonucleotides with nucleoside phosphoramidites on solid supports. The methods comprise the stepwise chain assembly of oligonucleotides on supports with 5?-acyl phosphoramidites. The synthesis cycles consist of a front end deprotection step which is conducted with a solution of a primary amine or a phenolate, a phosphoramidite coupling step with a 5?-acyl nucleoside phosphoramidite in the presence of an activator, a phosphite oxidation step and an optional capping step. The novel methods improve the quality of synthetic oligonucleotides due to the irreversibility of the front end deprotection step, which prevents the formation of deletion sequences, and due to the avoidance of acidic reagents in the synthesis cycles, which prevent the formation of depurination side products.

Abstract: A rack for electrical equipment is constructed for resistance to failure in earthquake conditions. The rack has a base and two legs extending upwardly from the base. A cross member spans between and interconnects the legs near their tops. A reinforcing strut on either the front or back flange of each leg forms, in combination with the flange a box beam element which greatly strengthens the flange of the leg. A brace attached to the underside of the cross member passes through an opening in the web of the leg and is rigidly attached to the reinforcing strut. The arrangement of the brace facilitates the mounting of EMI shielding on the rack. The rack also has a one-piece cover for closing the base of the rack.

Abstract: A support system for data transmission lines and the like, comprising a rail section supported generally horizontally, a splice for connecting the rail section and another structure, and a fastener for securing the splice to the rail section. Each rail section has a hollow box beam portion having a top wall, opposite side walls and a bottom wall. Each splice has a central part and first and second end parts, the first end part being receivable in an open end of the box beam portion of the rail section and the second end part being connectable to the structure. The central part of the splice has an outside width immediately adjacent the first end part of the splice essentially the same as the outside width of the rail section immediately adjacent its open end thereby to provide smooth junctures between the central part of the splice and the rail section at opposite sides of the rail section when the first end part of the splice is received in the rail section.

Abstract: The disclosure describes a sealing device for sealing two adjacent wireway sections positioned in an end-to-end relation. Each wireway section has a lower channel portion for receiving cable and a cover movable between an open position for placing cable in the wireway section and a closed position for covering the cable. The sealing device includes a sealing plate, a seal on a lower surface of the sealing plate, and a connector for connecting the sealing plate to the lower channel portion of at least one of the two wireway sections for pivotal movement of the sealing plate between an open position in which the covers of the wireway sections can be lifted, and a closed position in which the sealing plate extends transversely across the wireway sections over the covers with the seal in engagement with the covers. The sealing device also includes a quick-operating fastening device for releasably connecting the sealing plate to the lower channel portion of at least one of the two adjacent wireway sections.

Abstract: A support system for data transmission lines and the like includes a plurality of rail sections and a hub having a horizontal peripheral rim. A plurality of vertical holes are spaced at intervals around the rim for connecting the rail sections to the hub in positions in which the rail sections radiate out from the hub.

Abstract: A splice system having two rails to be connected end to end and splice member having a center part and a pair of end parts sized for insertion in open ends of the two rails. The rails to be connected end to end having first and second opposing walls and a hole in the first walls. The end parts of the splice members having threaded openings adapted to register with holes in the rails. The splice member is connected to the rails with bolts, the arrangement being such that, when the bolts are tightened, heads of the bolts outside the rails are drawn toward first walls of respective rails and end parts of the splice member inside the rails are pulled away from second walls of the rails and tight against the first walls of respective rails thereby to splice the rails together.