Patents Assigned to Signalgene
  • Subtractive amplification kit useful in the diagnosis of genetic disease mutation or variation
    Patent number: 6498024
    Abstract: A kit is provided for preferentially amplifying target RNA in a sample of tester RNA relative to non-target RNA in the sample, the kit driver sequences complementary to the non-target tester RNA under conditions where the driver sequences hybridize to the non-target RNA, a nucleic acid primer capable of hybridizing to the target RNA under conditions suitable for extension of the nucleic acid primer; and a promoter template capable of hybridizing to a DNA that is complementary to the target RNA under conditions suitable for extension of the complementary DNA such that a functional double promoter is formed.
    Type: Grant
    Filed: March 18, 1999
    Date of Patent: December 24, 2002
    Assignee: SignalGene Inc.
    Inventors: Lawrence T. Malek, Roy R. Sooknanan
  • Detection of CYP1A1, CYP3A4, CYP2D6 and NAT2 variants by PCR-allele-specific oligonucleotide (ASO) assay
    Patent number: 6183963
    Abstract: The present invention relates to a method of detecting genetic variation in individuals by PCR amplification of the locus of interest, transferring the resulting PCR products to a membrane filter, hybridizing with allele-specific-oligonucleotides (ASOs), and visualizing the results. Such a method is in the field of diagnostics, pharmacogenetics, cancer therapeutics, and drug metabolism. In particular, the present invention relates to the detection of genetic polymorphisms in gene encoding xenobiotics metabolizing enzymes CYP1A1, CYP2D6, CYP3A4, and NAT2.
    Type: Grant
    Filed: October 23, 1998
    Date of Patent: February 6, 2001
    Assignee: Signalgene
    Inventors: Daniel Sinnett, Damian Labuda