Abstract: Disclosed are methods for analyzing molecular binding events in which the formation of ligand/antiligand complexes can be directly detected in a mixture without requiring separation of the components of the mixture from each other by measuring bulk properties of the mixture (i.e., properties that have contributions from several or all of the components present in the mixture). Using these techniques, it is possible to screen libraries without labeling either the target antiligand or ligand. The invention also provides a method for determining the strength of ligand/antiligand binding by further analysis of the same signals.
Type:
Application
Filed:
July 17, 2002
Publication date:
January 22, 2004
Applicant:
Signature BioScience Inc. a corporation of California
Inventors:
Robert G. Chapman, Pejman Ghanouni, Igor Shmulevich, David Balaban, Joseph A. Heanue, Andrew P. May, Nikolai Sharkov