Abstract: Provided are methods, devices, and kits for the isolation and detection of one or more analytes of interest from a biological sample using microslit filter membranes. In various examples, the methods use capture particles and binding agents for specific recognition of one or more analytes of interest.
Type:
Grant
Filed:
August 16, 2018
Date of Patent:
March 19, 2024
Assignees:
SiMPore Inc., University of Rochester
Inventors:
James A. Roussie, James L. McGrath, Richard E. Waugh, Kilean S. Lucas, Joshua J. Miller
Abstract: Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereby forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.
Type:
Grant
Filed:
July 22, 2021
Date of Patent:
April 25, 2023
Assignee:
Simpore Inc.
Inventors:
Jared A. Carter, Akash S. Patel, Cassandra R. Walinski, James A. Roussie
Abstract: Provided are methods, devices, and kits for the isolation of extracellular vesicles using silicon nanomembranes. A method for EV isolation includes the steps of collecting a biofluid sample, contacting the biofluid sample with a pre-filtration membrane, thereby forming a first filtrate and a first retentate, optionally, washing the first retentate of the pre-filtration membrane, contacting the first filtrate from the pre-filtration membrane with a capture membrane, thereb forming a second filtrate and a second retentate, optionally, washing the second retentate, and eluting the second retentate from the capture membrane or lysing the second retentate to recover the contents.
Type:
Grant
Filed:
October 11, 2019
Date of Patent:
September 14, 2021
Assignee:
Simpore Inc.
Inventors:
Jared A. Carter, Akash S. Patel, Cassandra R. Walinski, James A. Roussie
Abstract: Provided are methods, devices, and kits for the isolation and enumeration of one or more components of interest within a liquid sample using microslit filter membranes. This disclosure relates to the enumeration of components within a sample of interest, and more particularly, the capture of such components by efficient isolation using microslit filters with high permeation capacity and precision molecular cut-off characteristics.
Abstract: Provided are monolithic structures comprising one or more suspended, nanoporous membranes that are in contact with one or more fluidic cavities, methods of making same, and exemplary uses of same. The monolithic structures can be formed using a transmembrane etch. The monolithic structures can be used, as examples, as filters and filtration modules in microfluidic devices, dialysis devices, and concentration devices in laboratory, industrial, and medical processes.
Type:
Grant
Filed:
October 31, 2016
Date of Patent:
June 2, 2020
Assignee:
SiMPore Inc.
Inventors:
Christopher C. Striemer, Joshua J. Miller, Jon-Paul S. Desormeaux, James A. Roussie
Abstract: Provided are nanoporous silicon nitride membranes and methods of making such membranes. The membranes can be part of a monolithic structure or free-standing. The membranes can be made by transfer of the nanoporous structure of a nanoporous silicon or silicon oxide film by, for example, reactive ion etching. The membranes can be used in, for example, filtration applications, hemodialysis applications, hemodialysis devices, laboratory separation devices, multi-well cell culture devices, electronic biosensors, optical biosensors, active pre-concentration filters for microfluidic devices.
Type:
Grant
Filed:
October 13, 2017
Date of Patent:
August 27, 2019
Assignee:
SiMPore, Inc.
Inventors:
Christopher C. Striemer, Jon-Paul DesOrmeaux
Abstract: Provided is a free-standing silicon oxide film that is under tensile stress. Also, provided are methods of making a free-standing silicon oxide film that is under tensile stress. The methods use low-power PECVD deposition of silicon oxide. Methods of imaging one or more objects (e.g., cells) using a free-standing silicon oxide film that is under tensile stress is also provided.
Type:
Grant
Filed:
November 19, 2014
Date of Patent:
April 17, 2018
Assignee:
SiMPore Inc.
Inventors:
Jon-Paul DesOrmeaux, Christopher C. Striemer
Abstract: Provided are nanoporous silicon nitride membranes and methods of making such membranes. The membranes can be part of a monolithic structure or free-standing. The membranes can be made by transfer of the nanoporous structure of a nanoporous silicon or silicon oxide film by, for example, reactive ion etching. The membranes can be used in, for example, filtration applications, hemodialysis applications, hemodialysis devices, laboratory separation devices, multi-well cell culture devices, electronic biosensors, optical biosensors, active pre-concentration filters for microfluidic devices.
Type:
Grant
Filed:
August 15, 2014
Date of Patent:
October 17, 2017
Assignee:
SiMPore, Inc.
Inventors:
Christopher C. Striemer, Jon-Paul DesOrmeaux
Abstract: The present invention is drawn to methods for facilitating fluid flow through the nanopores of membranes, i.e., through sub-micron pores. The present invention is also directed to one or more apparatus for such fluid flow, and for nanoporous membranes modified to facilitate such fluid flow.
Type:
Application
Filed:
October 1, 2010
Publication date:
July 5, 2012
Applicant:
SIMPORE, INC.
Inventors:
Thomas R. Gaborski, James L. McGrath, Richard D. Richmond, Christopher C. Striemer