Abstract: The present invention is directed to method of determining whether a subject is at risk of developing insulin resistance, particularly for advance alert of T2D and or CVD onset in obese and non-obese subject, by detecting the branched-chain amino acids (BCAAs) present in an urine sample (uBCAAs) of the subjects. The present invention also relates to a method for determining the need of a dietary/nutritional supplement for a subject involving said uBCAAs biomarkers. Finally, the invention is directed to kit comprising the biochemical network allowing the uBCAAs detection and process for the preparation of said biochemical networks as diagnostic biomarker.

Abstract: The present invention is directed to a method for the preparation of a non-living micro/nanoscale biosynthetic device capable of giving an information of a state of a system to analyse. Preferably, said device is used as an assay diagnostic, or to predict the risk, of a disease, or for the classification of mammal, preferably human pathologies. The invention also relates to a method for the identification and/or the quantification of a compound in a sample. Finally the present invention includes a kit comprising the biosynthetic device obtained by the method of the present invention.