Abstract: An assembly for cleaning the head portion of a stethoscope including a housing having a path of travel along which the head portion passes during cleaning. An activating assembly is operative to dispense a cleaning fluid on the head portion, via a dispenser assembly, concurrent to the head portion passing along the path of travel. Upon such activation, the cleaning fluid is delivered to an applicator assembly which distributes the cleaning fluid onto the head portion, facilitating the cleaning thereof. An included locking assembly is operative to prevent entry of or lockout the head portion relative to the path of travel, dependent on the status of the cleaning fluid.