Abstract: The invention relates to an infant positioning device for stabilizing and maintaining an infant in a fixed position for a lumbar puncture procedure. The device consists of a frame stand with openings for the infant's face and abdomen to support the infant in the proper position for the procedure and allow for unimpeded ventilation, a component of the frame to secure and stabilize the infant within the frame stand to prevent movement during the procedure, and an attachment stand to the frame that allows the frame to be free-standing. Vertically adjustable shoulder guards, tension-adjustable back strap, and tension adjustable head strap are provided for a custom fit.