Abstract: A three-dimensional positioning and motion-detecting system for human body and object status in mixed reality and a method thereof are disclosed. A virtualization computing device performs gesture recognition for scaling, moving or 3D rotating a virtual posture model, to make posture model aligning points of the virtual posture model correspond to the posture model positioning points one by one, a virtual-and-real display device displays the adjusted virtual posture model in real time, when the virtual posture model is fixed, the virtualization computing device recognizes a hand position on the physical environment image, and detects a motion on the hand position based on the posture model positioning points, to generate operating position information and hand motion information. The virtual-and-real display device displays virtual-and-real integration of operating position information, hand motion information and a physical space.