Abstract: A problem to be solved is to detect only free AIM in a biological sample containing complex AIM and free AIM. The problem can be solved by an immunoassay method for free AIM in a biological sample using an anti-AIM monoclonal antibody having a property of reacting with free AIM and not reacting with complex AIM, wherein the anti-AIM monoclonal antibody is an antibody binding to an epitope in the SRCR2 domain of human AIM.

Abstract: A problem to be solved is to further improve the specificity for free AIM of an antibody specifically reacting with free AIM and to diagnose NASH without imposing a burden on patients and medical staffs. The problem can be solved by an immunoassay method for free AIM in a biological sample containing complex AIM and free AIM, and the method comprises bringing the biological sample into contact with an antibody specifically reacting with free AIM in a presence of an anti-IgM antibody.

Abstract: A method and a diagnostic agent kit for detecting viral liver cancer having excellent sensitivity and specificity are provided. The problem can be solved by a method for detecting viral liver cancer, comprising measuring an amount of free AIM in a biological sample from a subject and comparing the measured amount of free AIM with a reference value.

Abstract: Disclosed is a method for measuring a blood coagulation activity, comprising: acquiring a parameter related to differentiation of a coagulation waveform in a blood specimen collected from a patient administered with (1) a polypeptide containing a sequence represented by SEQ ID NO: 1, or (2) a polypeptide containing a polypeptide having 95% or more identity with the sequence represented by SEQ ID NO: 1 and having an activity as a blood coagulation factor VIII; and acquiring an activity value of a blood coagulation factor VIII in the blood specimen based on the acquired parameter.

Abstract: A method for examining liver cancer or a method for assisting diagnosis of liver cancer that is superior in sensitivity and specificity, as well as a kit that can be used therefor is provided. The present invention provides a method for detecting liver cancer or a method for assisting diagnosis of liver cancer comprising a step of detecting or quantifying free AIM in a biological sample derived from a test subject, as well as a kit for examining or assisting diagnosis of liver cancer comprising an antibody that binds to free AIM.

Abstract: A method for examining liver cancer or a method for assisting diagnosis of liver cancer that is superior in sensitivity and specificity, as well as a kit that can be used therefor is provided. The present invention provides a method for detecting liver cancer or a method for assisting diagnosis of liver cancer comprising a step of detecting or quantifying free AIM in a biological sample derived from a test subject, as well as a kit for examining or assisting diagnosis of liver cancer comprising an antibody that binds to free AIM.

Abstract: Provided is a noninvasive method for determining the presence of a symptom of a hepatic disease such as nonalcoholic fatty liver disease with a higher diagnostic accuracy. A method for discriminate between nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH), the method comprising: (1) a step of measuring the quantities of marker molecules contained in blood collected from a subject; (2) a step of determining an index value from a normalized score calculated based on the quantities of the marker molecules belonging to the same group; and (3) a step of determining that the subject is possibly affected with NASH in the case that the index value is larger than a reference value.