Abstract: A segmented culture dish and imprinting system allows duplicate plates to be produced rapidly and without culture-to-culture overlap. The segmented culture dish and imprinting system includes a dish stamp, a first culture dish, and a second culture dish. The dish stamp may be placed on the first and/or the second culture dishes. The first and the second culture dishes have a key on an inside surface that ensures the orientation of cultures is always preserved, eliminating the need for markings on culture dishes.